{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1296450000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"10726.jpg","Size":120183,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"10726.tif","Size":42300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"10726.zip","Size":42164692,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355.","Oldenbourg R, Mei G (1995) J Microsc 180, 140-147.","Shribak M, Oldenbourg R (2003) Appl Opt 42, 3009-3017","LaFountain JR et al. (1992) Chromosoma 101, 425-441."],"Contributors":["James R. LaFountain","Rudolf Oldenbourg"],"PUBMED":["15385630","8537959","12790452"]},"BIOLOGICALPROCESS":{"onto_name":"male meiosis chromosome segregation","onto_id":"GO:0007060"},"PROCESSINGHISTORY":{"free_text":"4-frame algorithm"},"PARAMETERIMAGED":{"onto_name":"UV\/visible\/IR photons","onto_id":"FBbi:00000378"},"IMAGEDESCRIPTION":{"free_text":"Time series of anaphase A and B of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis. \nTime-lapse polarization microscopy using a Nikon \nMicrophot SA, equipped for liquid crystal polarized light microscopy \n(LC-PolScope, CRi, Woburn Massachusetts) 60x\/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 60 min using a QImaging Retigo EXi CCD camera. Raw images were processed \nusing 4-frame algorithm (Shribak and Oldenbourg, 2003). A movie of this time series is included in this grouped set."},"ITEMTYPE":[{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},{"free_text":"Processed image based on 4 recorded images"}],"CELLULARCOMPONENT":[{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic birefringence","onto_id":"FBbi:00000594"},"GROUP_ID":"11200","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"polarization microscopy","onto_id":"FBbi:00000248"},"DIMENSION":[{"Space":{"Image_size":678,"Pixel_size":{"unit":"nanometers","value":87},"axis":"X"}},{"Space":{"Image_size":518,"Pixel_size":{"unit":"nanometers","value":87},"axis":"Y"}},{"Time":{"unit":"seconds","value":30,"frame":"120"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Nephrotoma","onto_id":"NCBITaxon:46210"},{"free_text":"Nephrotoma suturalis"}],"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"free_text":"relative retardation (retardance) measurement"},"CELLTYPE":{"onto_name":"primary spermatocyte","onto_id":"CL:0000656"}}},"Citation":{"Title":"James R. LaFountain, Rudolf Oldenbourg (2011) CIL:10726, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10726","DOI":"doi:10.7295\/W9CIL10726"}}}