Ris, Hans; Korenberg, J.; Fawcett, Don W. (2021). CIL:11829, Cricetulus griseus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0MK6BP8

Transmission electron micrographs of whole metaphase chromosomes isolated from Chinese Hamster Ovary (CHO) cells showing the characteristic paired chromatids and centromeric constrictions.

• 2021

• Fawcett, Don W.
• Korenberg, J.
• Ris, Hans

The upper image shows a metaphase chromosome after mitotic cells were swollen in 0.075M KCl, fixed in 3:1 methanol:acetic acid, squashed in 50% acetic acid, stained with uranyl acetate and observed using transmission electron microscopy. The chromosomes consist of loops of ~30nm diameter chromatin fibers. The lower image shows chromosomes also isolated from CHO cells using the non-aqueous method of Wray and Stubblefield in the presence of calcium. Under these conditions, chromatin loops are ~50 nm in diameter.
These images were published as Figures 128 (upper, from H. Ris, Electron Microscopy, 1956) and 129 (lower, reprinted with permission by Academic Press, from H. Ris and J. Korenberg in Cell Biology, V2, Goldstein and Prescott, eds.) from Chapter 4 (Nucleus) of 'The Cell, 2nd Ed.' by Don W. Fawcett M.D. A PDF copy of the accompanying chapter is available on the ASCB's BioEDUCATE website.

Preparation: chromosome squash
Relation to intact cell: isolated subcellular component
Item type: film
Imaging mode: transmission electron microscopy (TEM); detection of electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: Image extracted from .pdf file of 'The Cell'
Data qualification: Processed

• Cell Image Library Group ID: 11180

• Cricetulus griseus

• Condensed nuclear chromosome, centromeric region
• Nuclear chromosome

• CHO
• Chromatin organization
• Chromosome organization
• Mitotic metaphase
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 11829

Doi: https://doi.org/10.6075/J0MK6BP8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL11829

Other resource

• Fawcett, Don W. "The Cell." 2nd ed., W.B. Saunders, 1981, ISBN 0721635849. https://www.ascb.org/career-development/teaching/don-fawcetts-the-cell/

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2022-08-12