{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1328418000},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":true},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"39555.jpg","Size":9525,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39555.zip","Size":1347327112,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26."],"Contributors":["David Ball","Jean Peccoud"],"PUBMED":["22046265"]},"BIOLOGICALPROCESS":[{"onto_name":"chromatin silencing at rDNA","onto_id":"GO:0000183"},{"onto_name":"nucleolus organization","onto_id":"GO:0007000"},{"onto_name":"regulation of exit from mitosis","onto_id":"GO:0007096"},{"onto_name":"cell cycle","onto_id":"GO:0007049"},{"onto_name":"cell division","onto_id":"GO:0051301"},{"onto_name":"mitosis","onto_id":"GO:0007067"}],"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"}],"IMAGEDESCRIPTION":{"free_text":"A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged NET1.  \n\nNET1 is a core subunit of the RENT complex that involves in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure.\n\nThese phase, gfp images are part of an image group that ranges from CIL:39551-39555. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"NET1 protein"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"RENT complex","onto_id":"GO:0030869"},{"onto_name":"nucleolus","onto_id":"GO:0005730"}],"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"GROUP_ID":"10630","MOLECULARFUNCTION":{"onto_name":"rDNA binding","onto_id":"GO:0000182"},"TECHNICALDETAILS":{"free_text":"Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x\/1.42 NA objective at 5 minute intervals.   Please see the microscopy section in the referenced manuscript for details for image analysis."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":[{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"}],"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"nanometers","value":213.4},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"nanometers","value":213.4},"axis":"Y"}},{"Time":{"unit":"microns","value":300,"frame":"61"}},{"Space":{"Image_size":21,"Pixel_size":{"unit":"microns","value":0.5},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}]}},"Citation":{"Title":"David Ball, Jean Peccoud (2012) CIL:39555, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39555","DOI":"doi:10.7295\/W9CIL39555"}}}