{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301457600},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13448.tif","Size":2100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13448.jpg","Size":15426,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"13448.zip","Size":2014582,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/1851\/"}],"PUBLISHED":["J Cell Biol. 187: 967-975. 2009"],"Contributors":["Christopher S. Wood","Karl R. Schmitz","Nicholas J. Bessman","Thanuja Gangi Setty","Kathryn M. Ferguson","Christopher G. Burd"],"PUBMED":["20026658"]},"BIOLOGICALPROCESS":[{"onto_name":"retrograde vesicle-mediated transport, Golgi to ER","onto_id":"GO:0006890"},{"onto_name":"protein localization in Golgi apparatus","onto_id":"GO:0034067"},{"onto_name":"protein transport","onto_id":"GO:0015031"},{"onto_name":"regulation of signal transduction","onto_id":"GO:0009966"}],"PROCESSINGHISTORY":{"onto_name":"constrained iterative deconvolution","onto_id":"FBbi:00000360"},"CELLLINE":{"free_text":"frq1-1"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Intracellular localization of GFP-GOLPH3 in S. cerevisiae frq1-1 temperature-conditional strain grown at restrictive temperature of 37C. In frq1-1 mutant after a 30 minute incubation at the restrictive temperature, GFP-GOLPH3 loses punctate localization and is mostly in the cytosol. The Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1. Vps74 is essential to maintain glycosyltransferases in the Golgi and results in this study reveal a previously unrecognized PtdIns4P-binding site in Vps74\/GOLPH3 family proteins and link Pik1 signaling to retention of Golgi-resident proteins. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1A GOLPH3\/frq1-1 37C panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1A include CIL#s 13439, 13440, 13441, 13442, 13443, 13444, 13445, 13446, 13447, 13448, 13458, 13459."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"cytosol","onto_id":"GO:0005829"},{"onto_name":"extrinsic to membrane","onto_id":"GO:0019898"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"Golgi membrane","onto_id":"GO:0000139"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"6596","MOLECULARFUNCTION":[{"onto_name":"phosphatidylinositol-4-phosphate binding","onto_id":"GO:0070273"},{"onto_name":"enzyme binding","onto_id":"GO:0019899"},{"onto_name":"calcium ion binding","onto_id":"GO:0005509"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":302,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"X"}},{"Space":{"Image_size":302,"Pixel_size":{"unit":"microns","value":0.0663},"axis":"Y"}},{"Space":{"Image_size":11,"Pixel_size":{"unit":"microns","value":0.4},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae S288c","onto_id":"NCBITaxon:559292"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"EGFP","onto_id":"FBbi:00000082"}}},"Citation":{"Title":"Christopher S. Wood, Karl R. Schmitz, Nicholas J. Bessman, Thanuja Gangi Setty, Kathryn M. Ferguson, Christopher G. Burd (2011) CIL:13448, Saccharomyces cerevisiae S288c. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13448","DOI":"doi:10.7295\/W9CIL13448"}}}