{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1314072000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"36746.zip","Size":10967658,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"36746.tif","Size":5500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36746.jpg","Size":1375589,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":{"onto_name":"digestive system process","onto_id":"GO:0022600"},"PROCESSINGHISTORY":[{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print of negative scanned for Photoshop"}],"PARAMETERIMAGED":[{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"elevation","onto_id":"FBbi:00000320"}],"IMAGEDESCRIPTION":{"free_text":"Conventional freeze-fractured images of DV-III membrane also suggests a transformation from a membrane with a smooth E-fracture face to one with characteristic large IMPs on its E-fracture face. The DV-III membrane resembles the lysosomal membrane which also has a moderate number of large IMPs on its E-fracture face. (For freeze-fracture study of DVs see Allen and Staehelin, J. Cell Biol. 89:9-20, 1981). TEM taken on 2\/16\/76 by R. Allen with JEM-100B operating at 80kV. Neg. 31,193X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":{"onto_name":"food vacuole","onto_id":"GO:0020020"},"RELATIONTOINTACTCELL":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"DIMENSION":[{"Space":{"Image_size":2100,"axis":"X"}},{"Space":{"Image_size":2604,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},"VISUALIZATIONMETHODS":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:36746, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36746","DOI":"doi:10.7295\/W9CIL36746"}}}