{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1306123200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"26533.zip","Size":792057,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"26533.tif","Size":800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"26533.jpg","Size":82706,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3112\/"}],"PUBLISHED":["J. Cell Biol. 2010. Vol. 190(4):663\u2013674"],"Contributors":["Chan-Soo Lee","Chang-Ki Choi","Eun-Young Shin","Martin Alexander Schwartz","Eung-Gook Kim"],"PUBMED":["PMID:20713598"]},"BIOLOGICALPROCESS":{"onto_name":"regulation of cell migration","onto_id":"GO:0030334"},"CELLLINE":{"onto_name":"NIH\/3T3","onto_id":"MCC:0000362"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were transfected with phosphomimetic constitutively active MLC mutants (MLCaa, green) and co-stained for βPIX (a Rac1\/Cdc42-specific GEF highly implicated in cell motility, red) and filamentous actin (blue). These findings help elucidate a functional link between MII and Rac1\/Cdc42 GTPases, which may regulate protrusion\/adhesion dynamics in migrating cells.  This image is the original data file from Fig. 7C,\"PDGF-induced dissociation of the MII\u2013βPIX  complex,\" in J. Cell Biol. 2010. Vol. 190(4):663\u2013674"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"lamellipodium","onto_id":"GO:0030027"},{"onto_name":"myosin II complex","onto_id":"GO:0016460"},{"onto_name":"actin filament","onto_id":"GO:0005884"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"}],"GROUP_ID":"8283","TECHNICALDETAILS":{"free_text":"Cells were cultured in DME (Invitrogen) supplemented with 10% fetal bovine serum and 100 U\/ml penicillin\/streptomycin (Invitrogen) at 37°C in a humidified 5% CO2 incubator. For transfections, cells in 60-mm-diameter dishes or on fibronectin-coated coverslips were incu- bated with a mixture of DNA and LipofectAMINE 2000 (Invitrogen) according to the manufacturer\u2019s instructions. The cDNA for MLC was subcloned into pCMV-myc (Takara Bio Inc.). Mutant constructs of MLC were generated using a QuikChange site-directed mutagenesis kit (Agilent Technologies) according to the manufacturer\u2019s protocol. In this myc-tagged MLC mutant, Ser1\/2 were replaced by alanines, and expressed in NIH3T3 cells.\nIn some experimental conditions, 16 h after replating onto fibronectin-coated coverslips, cells were treated with 50 ng\/ml PDGF for 20 min.  Cells were fixed 24\u201348 h after transfection using 3.7% paraformaldehyde in PBS for 15 min, permeabilized using 0.2% Triton X-100 in PBS for 2 min, blocked with 2% BSA in PBS, and co-stained for MLC (green), TRIO (red), and actin (blue). Images were captured by Zeiss LSM 710 confocal microscope with Plan-Apochromat 63X objective."},"DATAQUALIFICATION":{"free_text":"RAW"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":512,"axis":"X"}},{"Space":{"Image_size":512,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 546","onto_id":"FBbi:00000441"},{"onto_name":"phalloidin","onto_id":"FBbi:00000100"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26533, Mus musculus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil26533","DOI":"doi:10.7295\/W9CIL26533"}}}