{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1627079929},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"Drosophila antennae were prepared as described in Tsang et al. After cryofixation by high-pressure freezing and freeze-substitution in a cocktail containing uranyl acetate and glutaraldehyde, cryofixed antennae were rehydrated gradually at ice temperature. Rehydrated samples were then subjected to DAB labeling reaction and stained using a high-contrast en bloc staining protocol. Next, the samples were dehydrated for resin infiltration and embedded in Durcupan epoxy, followed by imaging with X-ray microscopy and then serial block-face scanning electron microscopy.\r\n<br>\r\nThe antenna was imaged using a Zeiss Gemini scanning electron microscope equipped with a Gatan 3View2XP and OnPoint backscatter detector. Images were acquired at 2.5 kV accelerating voltage with a 30 μm condenser aperture and 1.5 μsec dwell time; Z step size was 50 nm; pixel size was 5.06 nm; raster size was 12k x 16k; Z dimension was 658 sections, and local gas injection was set to 85%.Volume was aligned using cross correlation, segmented, and visualized using IMOD."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"ATTRIBUTION":{"OTHER":["Ir75c-GAL4>10XUAS-myc-APEX2-Orco"],"URLs":[{"Label":"Article","Href":"https:\/\/www.biorxiv.org\/content\/10.1101\/2021.04.28.441861v1.abstract"}],"Contributors":["Eric A Bushong","Mark H Ellisman","Cesar Nava Gonzales","Matthew Madany","Quintyn McKaughan","Chih-Ying Su","Tin Ki Tsang"]},"IMAGINGMODE":[{"onto_name":"serial block face SEM (SBFSEM)","onto_id":"FBbi:00000585"}],"IMAGEDESCRIPTION":{"free_text":"The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of these nanoscale characteristics for identified neurons are scarce. Taking advantage of the CryoChem method, which permits high-quality ultrastructural preservation of cryofixed and genetically labeled samples for volume EM, we acquired serial block-face scanning electron microscopy (SBEM) images of antennal tissues in which select ORNs expressed the APEX2 EM marker. These SEBEM datasets allow a systematic morphometric and morphological analysis of identified olfactory receptor neurons in Drosophila melanogaster."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Drosophila","onto_id":"NCBITaxon:7215"}],"CELLULARCOMPONENT":[{"onto_name":"vacuole","onto_id":"GO:0005773"},{"free_text":"sensillum lumen"},{"onto_name":"dendrite","onto_id":"GO:0030425"},{"free_text":"mitochondria"}],"CELLTYPE":[{"free_text":"olfactory receptor neuron"}],"GROUP_ID":"20079"},"Image_files":[{"File_type":"Jpeg","File_path":"54611.jpg","Size":1111827,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"54611.zip","Size":1106774,"Mime_type":"application\/zip"}],"Alternative_image_files":[{"File_type":"Mrc","URL_postfix":"\/media\/images\/54611\/IR75C-SBEM-Vol1.bin1.mrc","File_path":"IR75C-SBEM-Vol1.bin1.mrc","Size":132420453328,"Mime_type":"application\/octet-stream"}]}}}