{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1330318800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"40312.zip","Size":1151545,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"40312.tif","Size":1200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40312.jpg","Size":43496,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Proceedings National Academy of Sciences USA","Href":"http:\/\/www.pnas.org\/content\/100\/10\/5823.full.pdf+html?sid=400bc8e1-6feb-4a16-84a6-ae67ad1feeaa"}],"PUBLISHED":["J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827"],"Contributors":["Janet Cronshaw","Michael Matunis"],"PUBMED":["12730363"]},"BIOLOGICALPROCESS":[{"onto_name":"nucleus organization","onto_id":"GO:0006997"},{"onto_name":"nuclear transport","onto_id":"GO:0051169"},{"onto_name":"nuclear envelope organization","onto_id":"GO:0006998"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and the nucleoporin Nup358(red)within the nuclear envelope of HeLa cells. The two proteins show a close overlap with Nup358 slightly more distal to the midplane of the nuclear pore complex.\nOther images in this group show the ultrastructural effects of mutant ALADIN."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"nuclear envelope","onto_id":"GO:0005635"},{"onto_name":"nuclear pore","onto_id":"GO:0005643"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"11064","TECHNICALDETAILS":{"free_text":"HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Nup358 and observed with a Delta-vision deconvolution system.  \nSee: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"wide-field detection","onto_id":"FBbi:00000306"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":699,"axis":"X"}},{"Space":{"Image_size":549,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"EGFP","onto_id":"FBbi:00000082"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"Janet Cronshaw, Michael Matunis (2012) CIL:40312, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40312","DOI":"doi:10.7295\/W9CIL40312"}}}