CIL:40312, Homo sapiens, epithelial cell, cervical carcinoma
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- Cite This Work
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Cronshaw, Janet; Matunis, Michael (2021). CIL:40312, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XD10KZ
- Description
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Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and the nucleoporin Nup358(red)within the nuclear envelope of HeLa cells. The two proteins show a close overlap with Nup358 slightly more distal to the midplane of the nuclear pore complex.
Other images in this group show the ultrastructural effects of mutant ALADIN. - Date Issued
- 2021
- Researchers
- Methods
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HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Nup358 and observed with a Delta-vision deconvolution system.
See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827 - Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: wide-field detection; fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 594; EGFP
Data qualification: Processed - Series
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- Anatomy
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40312
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40312
- Cronshaw, J. M., & Matunis, M. J. (2003). The nuclear pore complex protein ALADIN is mislocalized in triple A syndrome. Proceedings of the National Academy of Sciences of the United States of America, 100(10), 5823–5827. https://doi.org/10.1073/pnas.1031047100
- J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827 https://www.ncbi.nlm.nih.gov/pubmed/?term=12730363
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- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12