{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307505600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13890.zip","Size":214657,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13890.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13890.jpg","Size":2694,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4053\/"}],"PUBLISHED":["J Cell Biol. (2011) 192: 599-614."],"Contributors":["Mauricio Valerio-Santiago","Fernando Monje-Casas"],"PUBMED":["21321099"]},"BIOLOGICALPROCESS":[{"onto_name":"cell cycle","onto_id":"GO:0007049"},{"onto_name":"regulation of exit from mitosis","onto_id":"GO:0007096"},{"onto_name":"mitotic cell cycle spindle orientation checkpoint","onto_id":"GO:0031578"},{"onto_name":"cell division","onto_id":"GO:0051301"},{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic sister chromatid segregation","onto_id":"GO:0000070"},{"onto_name":"microtubule-based process","onto_id":"GO:0007017"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"W303"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"3HA-Bfa1 (red) is generally localized to one spindle pole body (SPB) in wild-type cells in metaphase as determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Control image for CIL# 13891 in which constitutive targeting of Tem1 to both SPBs leads to symmetric SPB localization of Bfa1. Image is Fig 4C, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 4 include CIL# 13890, 13891, 13892, 13893."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"spindle pole body","onto_id":"GO:0005816"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"tubulin complex","onto_id":"GO:0045298"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"9034","MOLECULARFUNCTION":[{"onto_name":"GTPase activator activity","onto_id":"GO:0005096"},{"onto_name":"GTP binding","onto_id":"GO:0005525"},{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"nucleotide binding","onto_id":"GO:0000166"},{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"}],"TECHNICALDETAILS":{"free_text":"Cells (MATa bfa1::3HA-BFA1) grown in rich media with glucose were fixed for 15 min in 3.7% formaldehyde and 0.1 M potassium phosphate buffer, pH 6.4. Cells were then washed twice with 0.1 M potassium phosphate buffer, pH 6.4, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Fixed cells were digested with 0.1 mg\/ml zymolyase-100T (US Biological) and 1\/10 volume of glusulase (PerkinElmer) at 30C for 15 min, washed once, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Primary antibodies were anti-HA monoclonal antibody (HA.11; 1:500) and anti-tubulin (Abcam; 1:500). Secondary antibodies were: anti-mouse Cy3 (for HA) and anti-rat FITC (for tubulin). Cells were resuspended in DAPI (1 mg\/ml). Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x\/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"X"}},{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"HA peptide tag","onto_id":"FBbi:00000034"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"}]}},"Citation":{"Title":"Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13890, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13890","DOI":"doi:10.7295\/W9CIL13890"}}}