{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301284800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"13565.tif","Size":8500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13565.jpg","Size":64008,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2860\/"}],"PUBLISHED":["J Cell Biol. 189: 843-858. 2010."],"Contributors":["David J. Gill","Joanne Chia","Jamie Senewiratne","Frederic Bard"],"PUBMED":["PMID: 20498016"]},"BIOLOGICALPROCESS":[{"onto_name":"retrograde vesicle-mediated transport, Golgi to ER","onto_id":"GO:0006890"},{"onto_name":"COPI coating of Golgi vesicle","onto_id":"GO:0048205"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"IMAGEDESCRIPTION":{"free_text":"COP-I gamma1 (green) staining at the Golgi (GM130, a cis-Golgi marker) (red) in unstimulated HeLa cells. Helix Pomatia Lectin (HPL) (gray) staining is also shown. HPL binds various glycans but the Tn antigen in particular. The Tn antigen refers to terminal α-linked N-acetyl galactosamine residues (GalNAc) linked to Ser or Thr residues. HeLa cells were serum starved overnight in DME (noFBS). Cells were fixed for 10 min (4% paraformaldehyde) and permeabilized (0.2% Triton X-100). Primary antibody staining followed the manufacturer\u2019s instructions. Cells were subsequently stained for 15\u201330 min with secondary Alexa Fluor\u2013conjugated antibodies (Alexa 488 for anti-COP-I gamma1, Alexa 594 for anti-GM130). Hoechst (blue) and Alexa 647-conjugated-HPL were added during secondary antibody incubations. Cells were mounted onto glass slides using FluorSave (Merck) and imaged at room temperature using an inverted FluoView confocal microscope (model IX81; Olympus) with fluorescence excitation at 488 nm, 561 nm, and 633 nm and either a 60x objective (U Plan Super Apochromatic [UPLSAPO]; NA 1.35) or 100x objective (UPLSAPO; NA 1.40) using Immersol oil. Microscope coupled with a CCD camera (model FVII). Images were acquired and processed using Olympus FV10-ASW software. Image corresponds to Fig 6B, left panel, in J Cell Biol. 189: 843-858. 2010. Images in Fig 6 include CIL#s 13563, 13564, 13565, 13566, 13567, 13568, 13569, 13570."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi cisterna membrane","onto_id":"GO:0032580"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"Golgi-associated vesicle","onto_id":"GO:0005798"},{"onto_name":"COPI vesicle coat","onto_id":"GO:0030126"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"6490","MOLECULARFUNCTION":[{"onto_name":"structural molecule activity","onto_id":"GO:0005198"},{"onto_name":"N-acetylgalactosamine binding","onto_id":"GO:0046871"},{"onto_name":"protein binding","onto_id":"GO:0005515"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.062},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.062},"axis":"Y"}},{"Space":{"Image_size":1,"Pixel_size":{"unit":"microns","value":0.25},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 647","onto_id":"FBbi:00000447"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Helix pomatia agglutinin","onto_id":"FBbi:00000429"},{"free_text":"Hoechst"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"David J. Gill, Joanne Chia, Jamie Senewiratne, Frederic Bard (2011) CIL:13565, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13565","DOI":"doi:10.7295\/W9CIL13565"}}}