CIL: 54672
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- Cite This Work
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Lopez, Gabriela Salazar; Ross, Grace; Maserejian, Ariana; Coutinho-Budd, Jaeda (2024). CIL: 54672. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03B6003
- Description
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Confocal image of the CNS of a D. melanogaster in the third instar larval stage after Spz3 KD, 44uM from the ventral surface, depicting astrocytes (red), cortex glia (green) and neuronal nuclei (blue). Our study examined the breakdown of the spatial segregation between cortex glia and astrocyte after disruption of cortex glial morphology. After knockdown of Spz3, a secreted neurotrophin, we observed aberrant infiltration by astrocytes into the cortex.
- Date Issued
- 2024
- Researchers
- Methods
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Astrocyte-specific RFP expression was achieved using driven using the Gal4-UAS binary system and the driver alrm. Cortex-glia specific GFP was achieved using the lexA-lexAop binary system and the cortex-glia specific driver wrapper932i-LexA (Coutinho-Budd et al., 2017). The larval CNS was dissected in the third instar larval stage, fixed with methanol, stained for RFP, GFP and Elav (neuronal nuclei marker), and mounted in VectaShield reagent. Slides were imaged on an Innovative Imaging Innovations (3i) spinning disk confocal microscope equipped with a Yokogawa CSX-W1 using a 63X oil objective.
Staining: Astrocytes (red); Cortex glia (green); Neurons (blue) - Technical Details
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Imaging mode: fluorescence microscopy
Visualization methods: Spinning Disk Confocal Imaging - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 54672
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL54672
Source data
- License
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2024-01-12