{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1291266000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"10017.tif","Size":1100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"10017.jpg","Size":23467,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"10017.zip","Size":1049229,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Prabuddha Sengupta"]},"BIOLOGICALPROCESS":{"onto_name":"Golgi organization","onto_id":"GO:0007030"},"PROCESSINGHISTORY":{"free_text":"maximum projection"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Organization of Golgi and endoplasmic reticulum (ER) in a Human HeLa cell expressing Sialyl Transferase-EGFP (green) and Invariant Chain33-mRFP (red).  The Sialyl Transferase-EGFP is a Golgi marker and the Invariant Chain33-mRFP is retained in the ER.  There are two related files for this specimen:  the z series and the maximum projection.  This file is the maximum projection.\n\nZeiss Spinning Disc confocal used with with 220 ms exposure and a 63X oil objective."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"2406","MOLECULARFUNCTION":{"onto_name":"endoplasmic reticulum organization","onto_id":"GO:0007029"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.11},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.11},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"mRFP1","onto_id":"FBbi:00000527"},"CELLTYPE":[{"onto_name":"permanent cell line cell","onto_id":"CL:0000002"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Prabuddha Sengupta (2010) CIL:10017, Homo sapiens, permanent cell line cell, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil10017","DOI":"doi:10.7295\/W9CIL10017"}}}