{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1289365200},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"9058_web.mp4","Size":4313643,"Mime_type":"video\/mp4"},{"File_type":"Zip","File_path":"9058.zip","Size":24711292,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"9058.jpg","Size":135673,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Rudolf Oldenbourg","James R. LaFountain"],"PUBMED":["15385630","12790452"]},"BIOLOGICALPROCESS":{"onto_name":"spindle assembly involved in male meiosis II","onto_id":"GO:0007055"},"PROCESSINGHISTORY":{"free_text":"4-frame algorithm"},"PARAMETERIMAGED":{"onto_name":"UV\/visible\/IR photons","onto_id":"FBbi:00000378"},"IMAGEDESCRIPTION":{"free_text":"The events of meiosis II in a living insect spermatocyte beginning at the completion of prophase II through the comletion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x\/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 3 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. Raw images were processed using a 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white)."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"free_text":"Processed image based on 5 recorded images"}],"CELLULARCOMPONENT":[{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic birefringence","onto_id":"FBbi:00000594"},"GROUP_ID":"3782","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"polarization microscopy","onto_id":"FBbi:00000248"},"DIMENSION":[{"Space":{"Image_size":640,"Pixel_size":{"unit":"nanometers","value":87},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"nanometers","value":87},"axis":"Y"}},{"Time":{"unit":"seconds","value":30,"frame":"358"}}],"NCBIORGANISMALCLASSIFICATION":{"free_text":"Nephrotoma suturalis"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"free_text":"relative retardation (retardance) measurement"},"CELLTYPE":{"onto_name":"spermatocyte","onto_id":"CL:0000017"}}},"Citation":{"Title":"Rudolf Oldenbourg, James R. LaFountain (2010) CIL:9058, Nephrotoma suturalis, spermatocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil9058","DOI":"doi:10.7295\/W9CIL9058"}}}