{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1299474000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"17451.zip","Size":54302626,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"17451.jpg","Size":8845034,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"17451.tif","Size":54400000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"Contributors":["Gregory Antipa (San Francisco State University)"]},"BIOLOGICALPROCESS":[{"onto_name":"oral apparatus organization","onto_id":"GO:0032122"},{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"}],"CELLLINE":{"free_text":"Carolina Biological Supply Company, NC, U.S.A."},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"A transverse section near the mid region of the oral proboscis of Didinium. This micrograph shows the extrusive organelles of the mucocysts adjacent to the plasma membrane and the toxicysts. The pexicysts reside in the central region of the proboscis anterior to this section. A section toward the tip of the proboscis is found at (CIL:17452). Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. And details of the operation of this structure is available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 50nm thickness."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"oral apparatus","onto_id":"GO:0031912"},{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"free_text":"extrusomes"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11067","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4974,"Pixel_size":{"unit":"nanometers","value":1.7},"axis":"X"}},{"Space":{"Image_size":5458,"Pixel_size":{"unit":"nanometers","value":1.7},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Didinium nasutum","onto_id":"NCBITaxon:5997"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Gregory Antipa (San Francisco State University) (2011) CIL:17451, Didinium nasutum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil17451","DOI":"doi:10.7295\/W9CIL17451"}}}