{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301544000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"23038.zip","Size":4667230,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"23038.jpg","Size":1588000,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"23038.tif","Size":4700000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1996 Nov;135(4):991-1007"],"Contributors":["Tatyana M. Svitkina","Alexander B. Verkhovsky,","Gary G. Borisy"],"PUBMED":["8922382"]},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"REF-52"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},"IMAGEDESCRIPTION":{"free_text":"An REF-52 cytoskeleton with actin filaments decorated by myosin S1.  An intermediate filament associates with an actin filament bundle via plectin sidearms (10-nm immunogold labeling). Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon.  Image corresponds to Fig 10a from J Cell Biol. 1996 Nov;135(4):991-1007.  Image corresponds to Fig 10a in J Cell Biol. 1996 Nov;135(4):991-1007."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"free_text":"myosin S1"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"onto_name":"intermediate filament","onto_id":"GO:0005882"},{"free_text":"plectin"}],"RELATIONTOINTACTCELL":{"free_text":"platinum replica"},"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"8924","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1558,"axis":"X"}},{"Space":{"Image_size":2986,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus","onto_id":"NCBITaxon:10114"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"free_text":"tannic acid"},{"onto_name":"critical_point dried specimen","onto_id":"FBbi:00000581"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"free_text":"immunogold"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Tatyana M. Svitkina, Alexander B. Verkhovsky,, Gary G. Borisy (2011) CIL:23038, Rattus, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil23038","DOI":"doi:10.7295\/W9CIL23038"}}}