{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305000000},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"25378.tif","Size":3200000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"25378.jpg","Size":1230489,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"25378.zip","Size":6380238,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["El-Desouky Ammar","Saskia A. Hogenhout","L. R. Nault"]},"BIOLOGICALPROCESS":[{"onto_name":"viral transmission by vector","onto_id":"GO:0044009"},{"onto_name":"interspecies interaction between organisms","onto_id":"GO:0044419"}],"PROCESSINGHISTORY":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Electron micrograph of Maize mosaic virus (MMV, Rhabdoviridae) in secretory cells from the principal salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). MMV virions are bullet-shaped or bacilliform, 186-213 nm long and ca. 61 nm in diameter. In the principal salivary glands, MMV (arrows) buds through cytoplasmic membranes.  Abbreviations: ics, intercellular space;  SV, secretory vesicles. The accompanying image in this image group contains labels and scale bar. \n \nMethods: Dissected insect organs were processed for TEM by fixation in glutaraldehyde and osmium tetroxide and embedded in Spurr's medium. Thin sections were stained with uranyl acetate and lead citrate, and examined by a Philips-201 TEM.\n\nAssociated references: \nAmmar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.\nAmmar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41."},"ITEMTYPE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLULARCOMPONENT":{"onto_name":"vesicle","onto_id":"GO:0031982"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"7996","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"DIMENSION":[{"Space":{"Image_size":1626,"Pixel_size":{"unit":"microns","value":0.005},"axis":"X"}},{"Space":{"Image_size":1963,"Pixel_size":{"unit":"microns","value":0.005},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Maize mosaic virus","onto_id":"NCBITaxon:279896"},{"onto_name":"Peregrinus maidis","onto_id":"NCBITaxon:222432"}],"PREPARATION":[{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"secretory cell","onto_id":"CL:0000151"}}},"Citation":{"Title":"El-Desouky Ammar, Saskia A. Hogenhout, L. R. Nault (2011) CIL:25378, Maize mosaic virus, Peregrinus maidis, secretory cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25378","DOI":"doi:10.7295\/W9CIL25378"}}}