{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1301716800},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Mp4","File_path":"15806_web.mp4","Size":642010,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"15806.jpg","Size":63700,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"15806.zip","Size":4653184,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["George Von Dassow"]},"BIOLOGICALPROCESS":[{"onto_name":"mitotic anaphase","onto_id":"GO:0000090"},{"onto_name":"mitotic telophase","onto_id":"GO:0000093"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Embryo","onto_id":"FMA:69068"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Microtubules in sand dollar embryos expressing three tandem Cherry fluorophores fused to the ensconsin microtubule-binding domain (EMTB-3C).  Video demonstrates microtubule dynamics during process of mitosis.  Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14\u201317°C"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"spindle","onto_id":"GO:0005819"},{"onto_name":"astral microtubule","onto_id":"GO:0000235"},{"free_text":"ensconsin"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.137},"axis":"X"}},{"Space":{"Image_size":480,"Pixel_size":{"unit":"microns","value":0.137},"axis":"Y"}},{"Time":{"unit":"seconds","value":3,"frame":"179"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Dendraster excentricus","onto_id":"NCBITaxon:273752"},"PREPARATION":{"onto_name":"unfixed tissue","onto_id":"FBbi:00000014"},"VISUALIZATIONMETHODS":{"onto_name":"mCherryFP","onto_id":"FBbi:00000525"}}},"Citation":{"Title":"George Von Dassow (2011) CIL:15806, Dendraster excentricus. CIL. Dataset","ARK":"ark:\/b7295\/w9cil15806","DOI":"doi:10.7295\/W9CIL15806"}}}