{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303099200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13731.zip","Size":792129,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"13731.jpg","Size":43636,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"13731.tif","Size":800000,"Mime_type":"image\/tif"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3404\/"}],"PUBLISHED":["J Cell Biol, 191: 933-942, 2010."],"Contributors":["Seok Min Jin","Michael Lazarou","Chunxin Wang","Lesley A. Kane","Derek P. Narendra","Richard J. Youle"],"PUBMED":["21115803"]},"BIOLOGICALPROCESS":[{"onto_name":"respiratory electron transport chain","onto_id":"GO:0022904"},{"onto_name":"protein targeting to mitochondrion","onto_id":"GO:0006626"}],"PROCESSINGHISTORY":{"free_text":"brightness and contrast adjusted"},"CELLLINE":[{"onto_name":"HeLa","onto_id":"MCC:0000219"},{"free_text":"cervical carcinoma"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are permeabilized with 0.005% digitonin, the antibody to cyctochrome c (red), a mitochondrial matrix marker, is unable to reach and bind cytochrome c, but the antibody to Tom20 (white), a mitochondrial outer membrane marker, is now able to bind Tom20. Mito-YFP transfected HeLa cells were treated with CCCP (10µM) for 3 hrs, fixed with 4% paraformaldehyde and permeabilized with 0.005% digitonin\/PBS. Primary antibodies used were: anti-Cyt c mAb (BD) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4b, 5th row from top and is part of a differential permeabilization assay with and without CCCP that is further described in Fig 4b of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"mitochondrial outer membrane translocase complex","onto_id":"GO:0005742"},{"onto_name":"mitochondrial intermembrane space","onto_id":"GO:0005758"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"7051","MOLECULARFUNCTION":[{"onto_name":"electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity","onto_id":"GO:0045155"},{"onto_name":"heme binding","onto_id":"GO:0020037"},{"onto_name":"P-P-bond-hydrolysis-driven protein transmembrane transporter activity","onto_id":"GO:0015450"},{"onto_name":"oxidative phosphorylation uncoupler activity","onto_id":"GO:0017077"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"single-spot confocal microscopy","onto_id":"FBbi:00000252"},"DIMENSION":[{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.186},"axis":"X"}},{"Space":{"Image_size":512,"Pixel_size":{"unit":"microns","value":0.186},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"EYFP","onto_id":"FBbi:00000084"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":{"onto_name":"endothelial cell","onto_id":"CL:0000115"}}},"Citation":{"Title":"Seok Min Jin, Michael Lazarou, Chunxin Wang, Lesley A. Kane, Derek P. Narendra, Richard J. Youle (2011) CIL:13731, Homo sapiens, endothelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13731","DOI":"doi:10.7295\/W9CIL13731"}}}