Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13708, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P55M6D

YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl). HeLa cells stably expressing YFP-Parkin were transfected with control siRNA for 192 hours, treated with DMSO for 1 hour and then stained with TMRE, a cationic fluorescent dye that is readily sequestered by active mitochondria. Cells were imaged using an inverted microscope (LSM510 Meta; Carl Zeiss, Inc.) with a 63× 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the accompanying image browser (LSM; Carl Zeiss, Inc.). Image corresponds to Fig1f, top panels and is a control for other images in Fig1f in J Cell Biol. 191: 933-942, 2010. Images in Fig1f include CIL#s 13707, 13708, 13709.

• 2021

• Jin, Seok Min
• Kane, Lesley A.
• Lazarou, Michael
• Narendra, Derek P.
• Wang, Chunxin
• Youle, Richard J.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; compartmentalization of stain or label
Visualization methods: EYFP; probe for mitochondria; tetramethylrhodamine, ethyl ester (TMRE)
Processing history: brightness and contrast adjusted
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7051

• Homo sapiens

• Cervical carcinoma
• Cytosol
• Epithelial cell
• Mitochondrion
• Nucleus

• HeLa
• Mitochondrion degradation
• Protein binding
• Protein K48-linked ubiquitination
• Protein K63-linked ubiquitination
• Protein ubiquitination involved in ubiquitin-dependent protein catabolic process
• Ubiquitin-protein ligase activity
• Zinc ion binding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13708

Doi: https://doi.org/10.6075/J0P55M6D

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13708

Reference

• J Cell Biol. 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12