{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1327294800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39507.tif","Size":41800000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39507.jpg","Size":6180715,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39507.zip","Size":41745910,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["Richard Allen (University of Hawaii)"]},"BIOLOGICALPROCESS":[{"onto_name":"contractile vacuole organization","onto_id":"GO:0033298"},{"onto_name":"plasma membrane organization","onto_id":"GO:0007009"},{"onto_name":"microtubule-based process","onto_id":"GO:0007017"},{"onto_name":"detection of symbiont","onto_id":"GO:0009602"}],"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"CELLLINE":{"free_text":"Cambridge Culture Collection, Cambridge, England, # LB1690\/1"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"The contractile vacuole pore is supported on its cytosolic side by a coil of microtubules and other microtubules extend from the pore to the CV membrane. The plasma membrane and CV membrane cover the bottom of the pore. Bacteria reside in the chamber. TEM taken on 2\/14\/72 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 19,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"contractile vacuole","onto_id":"GO:0000331"},{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"contractile vacuolar membrane","onto_id":"GO:0031164"},{"onto_name":"contractile vacuole pore","onto_id":"GO:0031913"},{"onto_name":"microtubule associated complex","onto_id":"GO:0005875"},{"onto_name":"symbiont-containing vacuolar space","onto_id":"GO:0020004"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"10475","TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4530,"Pixel_size":{"unit":"nanometers","value":0.77},"axis":"X"}},{"Space":{"Image_size":4607,"Pixel_size":{"unit":"nanometers","value":0.77},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Vorticella convallaria","onto_id":"NCBITaxon:60850"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:39507, Vorticella convallaria, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39507","DOI":"doi:10.7295\/W9CIL39507"}}}