{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1332302400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"40556.zip","Size":56955122,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"40556.tif","Size":57000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"40556.jpg","Size":7989172,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Sci. 1995 Mar;108 ( Pt 3):1263-74."],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["7622609"]},"BIOLOGICALPROCESS":{"onto_name":"digestive system process","onto_id":"GO:0022600"},"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print from negative scanned for Photoshop."}],"PARAMETERIMAGED":[{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"}],"IMAGEDESCRIPTION":{"free_text":"Monoclonal antibody, E30-2-1, was raised to antigen D6 that specifically labels acidosomes and DV-II. There was no label on the nascent vacuole membrane or ciliary membranes. This cell had been incubated in 1.1µm latex beads for 3 minutes and fixed without a chase. There is some gold over the cytosol but the nascent vacuole lumen is essentially free of label. TEM taken on 7\/12\/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Adapted with permission."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"food vacuole","onto_id":"GO:0020020"},{"onto_name":"early phagosome","onto_id":"GO:0032009"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},"GROUP_ID":"11130","TECHNICALDETAILS":{"free_text":"To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4985,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"X"}},{"Space":{"Image_size":5712,"Pixel_size":{"unit":"nanometers","value":1.5},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in vitreous ice embedment","onto_id":"FBbi:00000089"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},{"free_text":"monoclonal antibody"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"gold","onto_id":"FBbi:00000202"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:40556, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil40556","DOI":"doi:10.7295\/W9CIL40556"}}}