7407
Alain Burette
purpose
PMCA1a in the adult rat brain
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512r&mpid=7407]]>
Raw image stack containing tiles for mosaic image in custom RTS format
/telescience/home/CCDB_DATA_USER.portal/P2061/Experiment_7259/Subject_7346/Tissue_7348/Microscopy_7407/PMCA1A-2K-HIPPO.IMG
1 #
oil
Nikon 60X oil
X
gelvatol
ebushong
1.40
PMCA1a labeling of hippocampus in adult rat
46 tiles
19 tiles
7407
PMCA1A-2K-HIPPO
RTS2000
MULTIPHOTON
MOSAIC
.24 um/pixels
512
.24 um/pixels
512
11
Emanuel E. Strehler
Richard J. Weinberg
Katherine A. Kenyon
National Institutes of Health (NS51769 EES)
Alain Burette
Localization of plasma membrane Ca2+ ATPases (PMCAs) in the rat brain
P2061
Kenyon, K. A., Filoteo, A. D., Bushong, E. A., Martone, M. E., Stehler, E. E., Weinberg, R. J. and Burette, A. C. Cellular and subcellular localization of PMCA1a in the rat brain. J. Comp. Neurol. in press
Large scale brain mosaic through the rat hippocampus immunolabeled for PMCA1a (red) and counterstained with Hoescht 33342 (green) to reveal cell nuclei. Note: hippocampus is flipped dorsal-ventral.
<![CDATA[http://ccdb.ucsd.edu/sand/main?event=rd&type=512v&mpid=7407]]>
Processed mosaic in tif format. CH1 (green): Hoescht; CH2 (red): Alexa 568 (PMCA1a)
/telescience/home/CCDB_DATA_USER.portal/P2061/Experiment_7259/Subject_7346/Tissue_7348/Microscopy_7407/PMCA1A-2K-HIPPO_t-0000_z-zproj_N_Combined_RGB.tif
TIFF
21511,8440,1
.24,.24,22
brain
central nervous system
Brain section was immunolabeled for PMCA1a using CR1a antibody from Emanuel Strehler (1:2000). Secondary antibody was Alexa Fluor 568. Some sections were then counterstained with DAPI (Invitrogen) or Hoescht 33342 (Invitrogen) to visualized all cell nuclei and/or with NeuroTrace 640/660 (Invitrogen) to selectively visualized somata of neuronal cells. The sections were coverslipped in Gelvatol.
Fluorescent IHC for PMCA1a
adult
male
Rattus norvegicus
Rat
Sprague Dawley
Brain
vibratome