{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329109200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39747.tif","Size":1000000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39747.jpg","Size":57588,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39747.zip","Size":939978,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"PLOSone","Href":"http:\/\/www.plosone.org\/article\/info%3Adoi%2F10.1371%2Fjournal.pone.0012768"}],"PUBLISHED":["A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768"],"Contributors":["A Matsuda","L Shao","J Boulanger","C Kervrann","PM Carlton","P Kner","D Agard","J Sedat"],"PUBMED":["20856676"]},"BIOLOGICALPROCESS":[{"free_text":"DNA packaging"},{"onto_name":"chromatin organization","onto_id":"GO:0006325"}],"PROCESSINGHISTORY":{"free_text":"PALM reconstruction"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"(C) Overview of cluster of metaphase chromosomes from a Drosophila embryo visualized using fluorescence microscopy showing the DAPI (blue) and histone (green) fluorescence. (A) PALM image of region in lower box in (A). Red arrowheads point to filamentous structures.  \nSee Fig 3 in: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768."},"ITEMTYPE":{"onto_name":"portrayed image","onto_id":"FBbi:00000266"},"CELLULARCOMPONENT":[{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},{"free_text":"chromatin"}],"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"GROUP_ID":"10721","TECHNICALDETAILS":{"free_text":"PALM was achieved using a custom wide-field microscope platform with 100x NA 1.4 objective lens or 150X TIRF NA 1.45 lens, yielding a pixel size at the CCD camera of 0.0792 or 0.0528 micrometers respectively.\nSee also: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"PALM (photoactivation localization microscopy)","onto_id":"FBbi:00000335"},"DIMENSION":[{"Space":{"Image_size":952,"axis":"X"}},{"Space":{"Image_size":329,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Drosophila melanogaster","onto_id":"NCBITaxon:7227"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"EGFP","onto_id":"FBbi:00000082"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"}],"CELLTYPE":{"onto_name":"early embryonic cell","onto_id":"CL:0000007"}}},"Citation":{"Title":"A Matsuda, L Shao, J Boulanger, C Kervrann, PM Carlton, P Kner, D Agard, J Sedat (2012) CIL:39747, Drosophila melanogaster, early embryonic cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39747","DOI":"doi:10.7295\/W9CIL39747"}}}