CIL:36776, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
CIL source metadata (JSON)
File Size |
|
File Format |
|
- Collection
- Cite This Work
-
Allen, Richard (2021). CIL:36776, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GX49H2
- Description
-
Posterior end of the buccal cavity with a nascent phagosome attached to the cytopharynx. The ribbed wall has ended and the cytopharynx is greatly reduced. The quadrulus goes to the end of the buccal cavity but the peniculi have ended, one or two rows of cilia at a time. The filamentous reticulum still covers the floor of the buccal cavity. Acidosomes are bound to the nascent phagosome and bundles of postoral microtubules lie close to the right edge of the developing phagosome. TEM taken on 1/31/74 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,100X. Bar = 1µm.
- Date Issued
- 2021
- Researcher
- Methods
-
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39161) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
-
Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Identifier
-
Samplenumber: 36776
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36776
Source data
- License
- Copyright
-
Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
-
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12