{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303185600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"21606.zip","Size":11984042,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"21606.tif","Size":5900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"21606.jpg","Size":2291347,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J. Cell Sci. 101:449-461, 1992"],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["1629255"]},"BIOLOGICALPROCESS":[{"onto_name":"endosome organization","onto_id":"GO:0007032"},{"onto_name":"cytoplasm organization","onto_id":"GO:0007028"},{"onto_name":"clathrin coat assembly","onto_id":"GO:0048268"}],"PROCESSINGHISTORY":[{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"},{"free_text":"Print of negative scanned for Photoshop"}],"PARAMETERIMAGED":[{"onto_name":"elastic scattering of electrons","onto_id":"FBbi:00000586"},{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"elevation","onto_id":"FBbi:00000320"}],"IMAGEDESCRIPTION":{"free_text":"Quick-freeze deep-etch rotary-shadowed replica of an early endosome with two clathrin-coated evaginations. Presumably these coated pits contain receptors for cargo that will be pinched off from the early endosomes and passed on through carrier vesicles to the late endosomal compartments. Early endosomes lie at the level of the intraciliary lattice and proximal ends of basal bodies, but internal to the striated bands. TEM taken on 6\/4\/91 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.25µm. Adapted with permission from J. Cell Sci. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/).A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12638) is available for quantitative analysis. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"coated pit","onto_id":"GO:0005905"},{"onto_name":"clathrin vesicle coat","onto_id":"GO:0030125"},{"onto_name":"early endosome","onto_id":"GO:0005769"}],"RELATIONTOINTACTCELL":[{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"GROUP_ID":"7156","DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"DIMENSION":[{"Space":{"Image_size":2146,"axis":"X"}},{"Space":{"Image_size":2736,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"},"VISUALIZATIONMETHODS":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2011) CIL:21606, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil21606","DOI":"doi:10.7295\/W9CIL21606"}}}