{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38720.tif","Size":900000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38720.jpg","Size":291231,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38720.zip","Size":871764,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/48\/3\/594.full.pdf+html?sid=6c2cff91-8475-4246-8ec9-caaf88a6a9f5"}],"PUBLISHED":["UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619."],"Contributors":["Ursula W. Goodenough","L. Andrew Staehelin"],"PUBMED":["4396088"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"CELLLINE":[{"free_text":"ac-5"},{"free_text":"ac-31"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were cryofixed, freeze-fractured, and surface replicas observed by transmission electron microscopy. This close-up of wildtype thylakoids shows the transition between the larger particles seen on the 'C' face of unstacked regions of thylakoids (Cu) and smaller particles on the stacked (Cs) face (region at right). See Fig 4 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid","onto_id":"GO:0009536"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"}],"SOURCEOFCONTRAST":{"onto_name":"differences in deposition of metal shadow","onto_id":"FBbi:00000601"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"GROUP_ID":"11387","TECHNICALDETAILS":{"free_text":"Isolated thylakoids were cryoprotected with glycerol, cryofixed, fractured at -100C, shadowed with Pt, and replicas prepared by carbon coating. Cleaned replicas were examined with a Philips 300 TEM. See Goodenough and Staehelin 1971 J Cell Biol 48:595-619"},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1596,"axis":"X"}},{"Space":{"Image_size":546,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlamydomonas reinhardtii","onto_id":"NCBITaxon:3055"},"PREPARATION":[{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},{"onto_name":"freeze_fracture\/freeze_etch","onto_id":"FBbi:00000419"}],"VISUALIZATIONMETHODS":{"onto_name":"shadowing and plating","onto_id":"FBbi:00000398"}}},"Citation":{"Title":"Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38720, Chlamydomonas reinhardtii. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38720","DOI":"doi:10.7295\/W9CIL38720"}}}