{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1293858000},"Data_type":{"Still_image":false,"Z_stack":false,"Video":true,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"11840.zip","Size":6930560,"Mime_type":"application\/zip"},{"File_type":"Mp4","File_path":"11840_web.mp4","Size":544555,"Mime_type":"video\/mp4"},{"File_type":"Jpeg","File_path":"11840.jpg","Size":44685,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol, 161:845-851, 2003"],"Contributors":["Torsten Wittmann","Gary M. Bokoch","Clare M. Waterman-Storer"],"PUBMED":["12796474"]},"BIOLOGICALPROCESS":{"onto_name":"microtubule polymerization","onto_id":"GO:0046785"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"Pt K1 (NBL-3)","onto_id":"MCC:0000388"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Microtubules in a PtK1 cell injected with the Pak inhibitory fragment PBD\/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60\/1.4 NA Plan Apo objective lens (Nikon).  Images were taken every 5 s and are played back at 15 frames\/s. For still images, see Fig. 4, A and C of associated paper (J Cell Biol, 161:845-851, 2003)."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":{"onto_name":"microtubule","onto_id":"GO:0005874"},"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"4105","MOLECULARFUNCTION":{"free_text":"PAK inhibition"},"DATAQUALIFICATION":{"free_text":"RAW;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":475,"Pixel_size":{"unit":"microns","value":0.179},"axis":"X"}},{"Space":{"Image_size":450,"Pixel_size":{"unit":"microns","value":0.179},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Potorous tridactylus","onto_id":"NCBITaxon:9310"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Torsten Wittmann, Gary M. Bokoch, Clare M. Waterman-Storer (2011) CIL:11840, Potorous tridactylus, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil11840","DOI":"doi:10.7295\/W9CIL11840"}}}