Microscopy product ID: 6674
Wide field mosaic of mouse hippocampus immunolabeled for pannexin 1 (green), GFAP (blue) and connexin 43 (red). The section was also counterstained with DAPI (not shown).
Full resolution data file
176 tiles (16x11) were digitally stitched together to generate a 3D mosaic montage of the mouse hippocampus labeled with three different antibodies (Green = Pannexin1 protein, Red = Connexin43 protein, Blue = GFAP protein within astrocytes). This zipped tar file contains 24 reconstructed mosaic images (file names labeled 00-23), one for each of the 24 optical Z-sections that were acquired with a step distance of 0.5 microns. Additionally, the zip file contains a mosaic 2D maximum intensity projection of all 24 Z-sections (file name containing "proj").
Down sampled data file
This image is a mosaic 2D maximum intensity projection of all 24 Z-sections. Voxel scale for this flattened projection of the mosaic hippocampus is 0.41 x 0.41 x 11.5 microns/pixel. The tiles in this image have been normalized, but the colors in the resulting image have not been adjusted.
- Research Team Head
- Creation Date
- Microscopy product: 2009-01-23. Experiment: 2008-12-22. Project: 2008-11-06.
- Scientific Name
- Technical Details
Product type: MOSAIC. Microscopy type: LASER SCANNING CONFOCAL. Instrument: Olympus Fluoview 1000
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
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