{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307505600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13875.zip","Size":285052,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13875.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13875.jpg","Size":6854,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4053\/"}],"PUBLISHED":["J Cell Biol. (2011) 192: 599-614."],"Contributors":["Mauricio Valerio-Santiago","Fernando Monje-Casas"],"PUBMED":["21321099"]},"BIOLOGICALPROCESS":[{"onto_name":"cell cycle","onto_id":"GO:0007049"},{"onto_name":"regulation of exit from mitosis","onto_id":"GO:0007096"},{"onto_name":"mitotic cell cycle spindle orientation checkpoint","onto_id":"GO:0031578"},{"onto_name":"cell division","onto_id":"GO:0051301"},{"onto_name":"mitosis","onto_id":"GO:0007067"},{"onto_name":"mitotic sister chromatid segregation","onto_id":"GO:0000070"},{"onto_name":"microtubule-based process","onto_id":"GO:0007017"},{"onto_name":"small GTPase mediated signal transduction","onto_id":"GO:0007264"},{"onto_name":"microtubule-based movement","onto_id":"GO:0007018"},{"onto_name":"establishment of mitotic spindle orientation","onto_id":"GO:0000132"},{"onto_name":"mitotic spindle elongation","onto_id":"GO:0000022"},{"onto_name":"nuclear migration along microtubule","onto_id":"GO:0030473"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"W303"},"PARAMETERIMAGED":[{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},{"onto_name":"optical path length gradient","onto_id":"FBbi:00000312"}],"IMAGEDESCRIPTION":{"free_text":"3HA-Bfa1 (red) localizes to both spindle pole bodies (SPBs) in anaphase in dyn1Δ cells with constitutive targeting of Tem1 to both SPBs. However, the constitutive presence of Tem1 on the SPBs impaired the spindle position checkpoint in the dyn1Δ cells. Anaphase was determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). A DIC image is also shown (hidden). Image is Fig S3 in J Cell Biol. (2011) 192: 599-614."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"spindle pole body","onto_id":"GO:0005816"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"},{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"tubulin complex","onto_id":"GO:0045298"},{"onto_name":"dynein complex","onto_id":"GO:0030286"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"},{"onto_name":"boundaries between regions with different refractive index","onto_id":"FBbi:00000599"}],"GROUP_ID":"9034","MOLECULARFUNCTION":[{"onto_name":"GTPase activator activity","onto_id":"GO:0005096"},{"onto_name":"GTP binding","onto_id":"GO:0005525"},{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"nucleotide binding","onto_id":"GO:0000166"},{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"},{"onto_name":"protein binding","onto_id":"GO:0005515"},{"onto_name":"ATP binding","onto_id":"GO:0005524"},{"onto_name":"microtubule motor activity","onto_id":"GO:0003777"}],"TECHNICALDETAILS":{"free_text":"Cells (MATa tem1::KanMX ura3::eGFP-CNM67\u2013TEM1::URA3 dyn1::URA3 bfa1::3HA-BFA1) grown in rich media at 14C for 24 hours were fixed for 15 min in 3.7% formaldehyde and 0.1 M potassium phosphate buffer, pH 6.4. Cells were then washed twice with 0.1 M potassium phosphate buffer, pH 6.4, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Fixed cells were digested with 0.1 mg\/ml zymolyase-100T (US Biological) and 1\/10 volume of glusulase (PerkinElmer) at 30C for 15 min, washed once, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Primary antibodies were anti-HA monoclonal antibody (HA.11; 1:500) and anti-tubulin (Abcam; 1:500). Secondary antibodies were: anti-mouse Cy3 (for HA) and anti-rat FITC (for tubulin). Cells were resuspended in DAPI (1 mg\/ml). Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x\/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},{"onto_name":"differential interference contrast microscopy","onto_id":"FBbi:00000245"}],"DIMENSION":[{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"X"}},{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"HA peptide tag","onto_id":"FBbi:00000034"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"}]}},"Citation":{"Title":"Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13875, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13875","DOI":"doi:10.7295\/W9CIL13875"}}}