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CIL:13738, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell

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Cite This Work

Hartman, Tiffiney R.; Zinshteyn, Daniel; Schofield, Heather K.; Nicolas, Emmanuelle; Okada, Ami; O'Reilly, Alana M. (2021). CIL:13738, Drosophila melanogaster, germ line cell, follicle cell, germ line stem cell, follicle stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GH9GQK

Description

Patched (Ptc, green) is expressed in most cells of the Drosophila wild-type germarium including follicle stem cells. Hedgehog (Hh, red) accumulates on the surface of the apical cells. Germ cells are identified using anti-Vasa (blue). Image is Fig 2A in J Cell Biol. 2010. 191: 943-952. Other images in Fig 2 include CIL# 13738, 13739, 13740, 13741, 13742).

Date Issued
  • 2021
Researchers
Methods

Fly ovaries were dissected and fixed as described previously (O'Reilly et al., 2008). Primary antibodies were 1:2,000 rabbit anti-Vasa (Hay et al., 1990); 1:100 goat anti-Hh (Santa Cruz Biotechnology, Inc.), 1:100 mouse anti-Ptc (DSHB). Secondary antibodies used were FITC, Cy3, and Cy5 conjugated to species-specific secondary antibodies (Jackson ImmunoResearch Laboratories, Inc.). Samples were mounted in Vectashield mounting medium. Images were collected at room temperature (22C) using 40× (1.25 NA) or 63× (1.4 NA) oil immersion lenses (Leica) on an upright microscope (DM 5000; Leica) coupled to a confocal laser scanner (TCS SP5; Leica). LAS AF SP5 software (Leica) was used for data acquisition. Images representing individual channels of single confocal slices from the center of each germarium were exported as TIFF files, and images were converted to figures using Photoshop software (Adobe).

Technical Details

Preparation: formaldehyde fixed tissue; detergent permeabilized
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: single-spot confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: primary antibody plus labeled secondary antibody; Fluorescein (FITC); Cy3; Cy5
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

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Identifier

Samplenumber: 13738

Doi: https://doi.org/10.6075/J0GH9GQK

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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

Last Modified

2022-08-12