{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1593056816},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. <br><br>\r\n4.7_Cell_4_GoldParticle_Segmentation is a multipage TIFF file illustrating the gold bead segmentation results (1.95×1.95×15 nm³ voxel size). Same as \u2018GoldParticles.am\u2019 file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview. The file contains 362 images, as all the planes that contained no segmentation data were removed.<br><br>\r\nThis file is a part of Dataset 4, comprised of files related to the Cell #4. The numbering order of the files reflects the order of operations performed during the analysis. Note: The data acquisition session was interrupted after frame #224, and later resumed as a separate imaging session. This resulted in two stack files, concatenated into a single multipage TIFF file during data analysis."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"ATTRIBUTION":{"OTHER":["Massachusetts Eye and Ear; Harvard Medical School","4.7_Cell_4_GoldParticle_Segmentation "],"URLs":[{"Label":"Scientific Data article","Href":"https:\/\/www.nature.com\/articles\/s41597-020-0509-4"},{"Label":"Nature Communications article","Href":"https:\/\/www.nature.com\/articles\/s41467-019-11712-w"}],"Contributors":["Maryna V. Ivanchenko","Marcelo Cicconet","Hoor Al Jandal","Xudong Wu","David P. Corey (<a href='http:\/\/corey.med.harvard.edu\/' target='_blank'>http:\/\/corey.med.harvard.edu\/<\/a>)","Artur A. Indzhykulian (<a href='https:\/\/ailab.hms.harvard.edu\/' target='_blank'>https:\/\/ailab.hms.harvard.edu\/<\/a>)"]},"IMAGINGMODE":[{"free_text":"Serial Electron Microscopy"},{"free_text":"Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)"}],"BIOLOGICALPROCESS":[{"free_text":"Native auditory Hair Cells at postnatal day 4"}],"IMAGEDESCRIPTION":{"free_text":"FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample."},"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"CELLULARCOMPONENT":[{"free_text":"Hair Cell stereocilia"},{"free_text":"Cuticular plate"},{"free_text":"Mitochondria "},{"free_text":"Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)"}],"VISUALIZATIONMETHODS":[{"free_text":"Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid)"},{"free_text":"Immunogold labeling with 10 nm gold beads"}],"CELLTYPE":[{"free_text":"Inner Ear Outer Hair Cell"}],"GROUP_ID":"20004"},"Image_files":[{"File_type":"Jpeg","File_path":"50710.jpg","Size":7050,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"50710.zip","Size":2513203771,"Mime_type":"application\/zip"}]},"Citation":{"Title":"Maryna V. Ivanchenko, Marcelo Cicconet, Hoor Al Jandal, Xudong Wu, David P. Corey, Artur A. Indzhykulian (2019). FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 4.7_Cell_4_GoldParticle_Segmentation. CIL","ARK":"ark:\/b7295\/w9cil50710","DOI":"doi:10.7295\/W9CIL50710"}}}