{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1593056818},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"CORE":{"TECHNICALDETAILS":{"free_text":"Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. <br><br>\r\n6.2_Cell_6_Stack_Aligned_Cropped is a multipage TIFF file containing aligned, then cropped images of Cell#6, which were cropped to contain only the relevant volume, and reduce the file size. No filters were used to analyze this dataset. The file contains 324 images, as all the planes that contained no relevant data were removed.<br><br>\r\nThis file is a part of Dataset 6, comprised of files related to the Cell #6 of current study. The numbering order of the files reflects the order of operations performed during the analysis. The raw imaging data related to this cell was deposited within Dataset 1 (CIL:50680)."},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"ATTRIBUTION":{"OTHER":["Massachusetts Eye and Ear; Harvard Medical School","6.2_Cell_6_Stack_Aligned_Cropped"],"URLs":[{"Label":"Scientific Data article","Href":"https:\/\/www.nature.com\/articles\/s41597-020-0509-4"},{"Label":"Nature Communications article","Href":"https:\/\/www.nature.com\/articles\/s41467-019-11712-w"}],"Contributors":["Maryna V. Ivanchenko","Marcelo Cicconet","Hoor Al Jandal","Xudong Wu","David P. Corey (<a href='http:\/\/corey.med.harvard.edu\/' target='_blank'>http:\/\/corey.med.harvard.edu\/<\/a>)","Artur A. Indzhykulian (<a href='https:\/\/ailab.hms.harvard.edu\/' target='_blank'>https:\/\/ailab.hms.harvard.edu\/<\/a>)"]},"IMAGINGMODE":[{"free_text":"Serial Electron Microscopy"},{"free_text":"Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)"}],"BIOLOGICALPROCESS":[{"free_text":"Native auditory Hair Cells at postnatal day 4"}],"IMAGEDESCRIPTION":{"free_text":"FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample."},"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"}],"CELLULARCOMPONENT":[{"free_text":"Hair Cell stereocilia"},{"free_text":"Cuticular plate"},{"free_text":"Mitochondria "},{"free_text":"Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)"}],"VISUALIZATIONMETHODS":[{"free_text":"Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid)"},{"free_text":"Immunogold labeling with 10 nm gold beads"}],"CELLTYPE":[{"free_text":"Inner Ear Outer Hair Cell"}],"GROUP_ID":"20006"},"Image_files":[{"File_type":"Jpeg","File_path":"50720.jpg","Size":2537182,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"50720.zip","Size":3244223094,"Mime_type":"application\/zip"}]},"Citation":{"Title":"Maryna V. Ivanchenko, Marcelo Cicconet, Hoor Al Jandal, Xudong Wu, David P. Corey, Artur A. Indzhykulian (2019). FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 6.2_Cell_6_Stack_Aligned_Cropped. CIL","ARK":"ark:\/b7295\/w9cil50720","DOI":"doi:10.7295\/W9CIL50720"}}}