{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1302580800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13655.zip","Size":4340431,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13655.tif","Size":4400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13655.jpg","Size":97209,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/2906\/"}],"PUBLISHED":["J Cell Biol. 190: 893-910. 2010."],"Contributors":["Hung-Hsiang Huang","Pamela Stanley"],"PUBMED":["20805325"]},"BIOLOGICALPROCESS":[{"onto_name":"protein N-linked glycosylation via asparagine","onto_id":"GO:0018279"},{"onto_name":"carbohydrate metabolic process","onto_id":"GO:0005975"}],"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"HeLa cells transiently expressing GlcNAcT-1-HA-KDEL were fixed, immunolabeled for GlcNAcT-1-HA-KDEL (red) and endogenous alpha-mannosidase II (ManII, green), and examined by fluorescence microscopy. Endogenous ManII displayed different staining patterns, depending upon the level of expression of GlcNAcT-1-HA-KDEL. In this image, ManII is mislocalized to the ER. Other images in Fig 6 show ManII localization to the Golgi (CIL# 13656), or cells in which ManII protein is lost completely (CIL# 13657). Transfected HeLa cells were fixed in 3% paraformaldehyde, blocked with 0.2% Triton X-100, 1% FBS, 0.5% BSA in PBS with Ca[2+] and Mg[2+], followed by incubation in primary antibodies (anti\u2013human ManII rabbit pAb and anti-HA mouse mAb, HA.11 (Covance)) and secondary antibodies (Alexa 568 and Alexa 488) and DAPI (blue) to stain nuclei. Cells were mounted using Fluoromount (SouthernBiotech). Immunofluorescent images were digitally acquired on an inverted microscope (Axiovert 200M; Carl Zeiss, Inc.) coupled to a 12-bit cooled charge-coupled device camera (AxioCam MRm Rev. 3; Carl Zeiss, Inc.) controlled by Axiovision software (AxioVs40, version 4.7.2.0; Carl Zeiss, Inc.), using a 100x\/1.3 NA oil immersion objective (EC Plan-NeoFluar; Carl Zeiss, Inc.), and saved as tiff files (1388 × 1040, 8 bit). All pictures were treated identically using Adobe Photoshop to remove background and adjust contrast. This image corresponds to Figure 6D, top panel (merge) in J Cell Biol. 190: 893-910, 2010. Images in Fig 6 include CIL#s 13655, 13656, 13657."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"Golgi apparatus","onto_id":"GO:0005794"},{"onto_name":"integral to membrane","onto_id":"GO:0016021"},{"onto_name":"Golgi membrane","onto_id":"GO:0000139"},{"onto_name":"endoplasmic reticulum","onto_id":"GO:0005783"},{"onto_name":"endoplasmic reticulum-Golgi intermediate compartment","onto_id":"GO:0005793"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"6874","MOLECULARFUNCTION":[{"onto_name":"alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity","onto_id":"GO:0003827"},{"onto_name":"mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity","onto_id":"GO:0004572"},{"onto_name":"zinc ion binding","onto_id":"GO:0008270"}],"DATAQUALIFICATION":{"free_text":"PROCESSED;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"},"DIMENSION":[{"Space":{"Image_size":1388,"Pixel_size":{"unit":"microns","value":169.333},"axis":"X"}},{"Space":{"Image_size":1040,"Pixel_size":{"unit":"microns","value":169.333},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":[{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"HA peptide tag","onto_id":"FBbi:00000034"}],"CELLTYPE":[{"onto_name":"epithelial cell","onto_id":"CL:0000066"},{"free_text":"cervical carcinoma"}]}},"Citation":{"Title":"Hung-Hsiang Huang, Pamela Stanley (2011) CIL:13655, Homo sapiens, epithelial cell, cervical carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13655","DOI":"doi:10.7295\/W9CIL13655"}}}