{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1309406400},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"13608.zip","Size":1493419,"Mime_type":"application\/zip"},{"File_type":"OME_tif","File_path":"13608.tif","Size":1500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"13608.jpg","Size":2887,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["Bassett et al. 2010 J Cell Biol 190:177-185"],"Contributors":["Emily Bassett","Stacey Wood","Kevan Salimian","Sandya Ajith","Daniel Foltz","Ben Black"],"PUBMED":["20643881"]},"BIOLOGICALPROCESS":{"onto_name":"mitosis","onto_id":"GO:0007067"},"PROCESSINGHISTORY":{"onto_name":"3D-deconvolution","onto_id":"FBbi:00000357"},"CELLLINE":{"free_text":"PD-NC4"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Behavior of a patient-derived human fibroblast line (PD-NC4) containing a mutated chromosome 4 in which the normal centromere is epigenetically silenced and a neo-centromere has formed 25 Mbp down the q arm at a site that lacks centromere repeats. Shown is a z-series of the PD-NC4 chromosome from a metaphase spread stained for CENP-B (red), centromere (ACA, green) and DNA (DAPI blue).  See Fig 1D in Bassett et al. 2010. Epigenetic centromere specification directs aurora B accumulation but is insufficient to efficiently correct mitotic errors. J Cell Biol 190:177-185."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"CELLULARCOMPONENT":[{"onto_name":"nuclear chromosome","onto_id":"GO:0000228"},{"onto_name":"chromosome, centromeric region","onto_id":"GO:0000775"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"},{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"}],"GROUP_ID":"8693","TECHNICALDETAILS":{"free_text":"Cells were metaphase-arrested with nocodazole, swollen in 75 mM KCl, centrifuged onto glass slides, and fixed with formaldehyde before processing for indirect immunofluorescence. Digital z-series images were captured with an ORCA AG CCD mounted on  DMI6000B Leica microscope using a 100X 1.4 NA objective lens and subsequently processed by deconvolution."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"DIMENSION":[{"Space":{"Image_size":101,"axis":"X"}},{"Space":{"Image_size":91,"axis":"Y"}},{"Space":{"Image_size":20,"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"},{"onto_name":"Cy5","onto_id":"FBbi:00000450"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Emily Bassett, Stacey Wood, Kevan Salimian, Sandya Ajith, Daniel Foltz, Ben Black (2011) CIL:13608, Homo sapiens, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13608","DOI":"doi:10.7295\/W9CIL13608"}}}