Microscopy product ID: 7796
2D Image
Description | Merged fluorescent and DIC image of MDCK cells expressing Cx43-4C309/337 (green) |
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Description | Optical section/time series in Olympus Fluoview .oif format. Each time point represents 11 z slices from the fluorescent channel and a DIC image. |
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Description | Time-lapse imaging of FlAsH-labeled MDCK cells expressing Cx43-4C309/337 (green) showing their rearrangement and fate during and after mitosis. FlAsH fluorescence excitation z series along with a transmitted DIC image were recorded every 6 min for a total duration of about 5 h. 3D volume reconstructions from confocal image stacks over time are displayed at a rate of two frames per minute. |
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Description | File containing the merged fluorescent-DIC imaging for each time point, where the fluorescent image is a maximum intensity projection of the z series collected at each time point. Each time point is represented as a "slice" in the volume in IMOD format. |
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Description | Annnotated time-lapse animation of FlAsH-labeled MDCK cells expressing Cx43-4C309/337 (green) showing their rearrangement and fate during and after mitosis. FlAsH fluorescence excitation z series along with a transmitted DIC image were recorded every 6 min for a total duration of about 5 h. 3D volume reconstructions from confocal image stacks over time are displayed at a rate of two frames per minute. Supplemental movie 2 from Boassa et al. (2010). |
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Boassa, Daniela; Sosinsky, Gina; Lampe, Paul; Solan, Joell (2017). Microscopy product ID: 7796. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0J10300
- Date Issued
- 2017
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- Technical Details
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Product type: TIME SERIES. Microscopy type: LASER SCANNING CONFOCAL. Instrument: Olympus Fluoview 1000
- Funding
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NIH GM072881 and GM065937 awarded to Gina Sosinky, NIH GM55632 awarded to Paul Lampe
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- Anatomy
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- No linguistic content; Not applicable
- Related Resources
- Boassa D, Solan JL, Papas A, Thornton P, Lampe PD, Sosinsky GE (2010). Trafficking and recycling of the connexin43 gap junction protein during mitosis. Traffic, 11(11):1471-1486. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3272544/; Traffic. https://doi.org/10.1111/j.1600-0854.2010.01109.x
- Microscopy product 7796 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB7796
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Creative Commons Attribution 4.0 International Public License
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- UC Regents
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22