{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1326862800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39306.tif","Size":60100000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39306.jpg","Size":8235130,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39306.zip","Size":60019150,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"PUBLISHED":["J Cell Biol. 1971 Apr;49(1):1-20."],"Contributors":["Richard Allen (University of Hawaii)"],"PUBMED":["4102004"]},"BIOLOGICALPROCESS":{"onto_name":"cortical cytoskeleton organization","onto_id":"GO:0030865"},"PROCESSINGHISTORY":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"PARAMETERIMAGED":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"IMAGEDESCRIPTION":{"free_text":"This EM features the infraciliary lattice which forms a continuous network at the level of the proximal ends of basal bodies where the kinetodesmal fibers are connected to the posterior basal bodies of a pair. This system is separated by a small gap from the more superficially located striated bands. The gap has no apparent fibers connecting these two systems. This network forms a highly porous boundary between the ectoplasm and the endoplasm. Electron dense posts are located at each branching site of this network. It is now known that this network is composed of centrin, a contractile protein also found in centrosomes of higher animals including man. TEM taken on 12\/18\/68 by R. Allen with Philips 300. Neg. 16,000X. Published in J. Cell Biol. 49:1-20, 1971."},"ITEMTYPE":[{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},{"onto_name":"illumination by electrons","onto_id":"FBbi:00000273"}],"CELLULARCOMPONENT":[{"onto_name":"plasma membrane","onto_id":"GO:0005886"},{"onto_name":"cell cortex","onto_id":"GO:0005938"}],"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"SOURCEOFCONTRAST":[{"onto_name":"electron density","onto_id":"FBbi:00000315"},{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"}],"TECHNICALDETAILS":{"free_text":"Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http:\/\/www5.pbrc.hawaii.edu\/allen\/)."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"detection of electrons","onto_id":"FBbi:00000375"},{"onto_name":"film","onto_id":"FBbi:00000303"}],"DIMENSION":[{"Space":{"Image_size":4772,"Pixel_size":{"unit":"nanometers","value":0.94},"axis":"X"}},{"Space":{"Image_size":6288,"Pixel_size":{"unit":"nanometers","value":0.94},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Paramecium multimicronucleatum","onto_id":"NCBITaxon:44030"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"osmium tetroxide fixed tissue","onto_id":"FBbi:00000012"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"},{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"}],"VISUALIZATIONMETHODS":[{"onto_name":"stain with broad specificity","onto_id":"FBbi:00000415"},{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":[{"onto_name":"cell by organism","onto_id":"CL:0000004"},{"onto_name":"eukaryotic cell","onto_id":"CL:0000255"},{"free_text":"Eukaryotic Protist"},{"free_text":"Ciliated Protist"}]}},"Citation":{"Title":"Richard Allen (University of Hawaii) (2012) CIL:39306, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39306","DOI":"doi:10.7295\/W9CIL39306"}}}