{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1303963200},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Zip","File_path":"27832.zip","Size":5152719102,"Mime_type":"application\/zip"},{"File_type":"Jpeg","File_path":"27832.jpg","Size":522100,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"Contributors":["Yumi Uetake","Greenfield Sluder"]},"BIOLOGICALPROCESS":[{"free_text":"cell motility"},{"free_text":"cell morphology"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"hTERT-RPE1"},"PARAMETERIMAGED":{"onto_name":"elastic scattering of photons","onto_id":"FBbi:00000587"},"IMAGEDESCRIPTION":{"free_text":"Time lapse series showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1  using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671). \nA movie prepared from this time series is grouped in the Cell Image library."},"ITEMTYPE":{"onto_name":"charge coupled device (CCD)","onto_id":"FBbi:00000294"},"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"differences in amount of elastic light scattering","onto_id":"FBbi:00000600"},"GROUP_ID":"8518","TECHNICALDETAILS":{"free_text":"Cells were cultured on glass coverslips in 10% FBS, DMEM\/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"phase contrast microscopy","onto_id":"FBbi:00000247"},"DIMENSION":[{"Space":{"Image_size":1344,"Pixel_size":{"unit":"microns","value":0.645},"axis":"X"}},{"Space":{"Image_size":1024,"Pixel_size":{"unit":"microns","value":0.645},"axis":"Y"}},{"Time":{"unit":"seconds","value":180,"frame":"1868"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"living tissue","onto_id":"FBbi:00000025"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"},"CELLTYPE":{"free_text":"retinal pigment epithelium"}}},"Citation":{"Title":"Yumi Uetake, Greenfield Sluder (2011) CIL:27832, Homo sapiens, retinal pigment epithelium. CIL. Dataset","ARK":"ark:\/b7295\/w9cil27832","DOI":"doi:10.7295\/W9CIL27832"}}}