{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1320379200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38715.tif","Size":1500000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38715.jpg","Size":356209,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38715.zip","Size":1434804,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Journal of Cell Biology","Href":"http:\/\/jcb.rupress.org\/content\/48\/3\/594.full.pdf+html?sid=6c2cff91-8475-4246-8ec9-caaf88a6a9f5"}],"PUBLISHED":["UW Goodenough and LA Staehelin (1971) Structural differentiation of stacked and unstacked chloroplast membranes. J Cell Biol 48:595-619."],"Contributors":["Ursula W. Goodenough","L. Andrew Staehelin"],"PUBMED":["4396088"]},"BIOLOGICALPROCESS":[{"onto_name":"photosynthesis","onto_id":"GO:0015979"},{"onto_name":"organelle organization","onto_id":"GO:0006996"}],"CELLLINE":[{"free_text":"ac-5"},{"free_text":"ac-31"}],"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Isolated chloroplast membranes of the unicellular photosynthetic alga Chlamydomonas reinhardtii were fixed, sectioned, and examined by transmission electron microscopy. Under the conditions used here, with the ac-31 mutant, thylakoids are completely unstacked.  See Fig 19 in Goodenough and Staehelin 1971 J Cell Biol 48:595-619."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"chloroplast","onto_id":"GO:0009507"},{"onto_name":"plastid","onto_id":"GO:0009536"},{"onto_name":"plastid thylakoid membrane","onto_id":"GO:0055035"}],"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"RELATIONTOINTACTCELL":{"onto_name":"microtome-sectioned tissue","onto_id":"FBbi:00000029"},"GROUP_ID":"11387","TECHNICALDETAILS":{"free_text":"Isolated chloroplast membranes were fixed with glutaraldehyde, post fixed with osmium tetroxide, dehydrated, embedded in epoxy resin, and thin sections cut, stained and examined with a Hitachi HU-11C TEM."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1374,"axis":"X"}},{"Space":{"Image_size":1044,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Chlamydomonas reinhardtii","onto_id":"NCBITaxon:3055"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":[{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}]}},"Citation":{"Title":"Ursula W. Goodenough, L. Andrew Staehelin (2011) CIL:38715, Chlamydomonas reinhardtii. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38715","DOI":"doi:10.7295\/W9CIL38715"}}}