{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331355600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39727.tif","Size":8700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39727.jpg","Size":1109012,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Mount Holyoke College"],"Contributors":["Dee Lauzon","Sue Lancelle","Marian Rice"]},"BIOLOGICALPROCESS":{"onto_name":"regulation of cardiac muscle contraction","onto_id":"GO:0055117"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of mouse cardiac muscle (ventricle). The image illustrates the myofibrils as well as a sarcomere in the area of fibrils between the two Z-lines.  Z-lines link actin filaments of one sarcomere to the next, while the sarcomere is responsible for cardiac muscle contractions."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"sarcomere","onto_id":"GO:0030017"},{"onto_name":"myofibril","onto_id":"GO:0030016"},{"onto_name":"Z disc","onto_id":"GO:0030018"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"10969","TECHNICALDETAILS":{"free_text":"Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in  0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr\u2019s resin.  Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1800,"axis":"X"}},{"Space":{"Image_size":1602,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"free_text":"ethanol dehydration"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"cardiac muscle cell","onto_id":"CL:0000746"}}},"Citation":{"Title":"Dee Lauzon, Sue Lancelle, Marian Rice (2012) CIL:39727, Mus musculus, cardiac muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39727","DOI":"doi:10.7295\/W9CIL39727"}}}