{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1331355600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39760.tif","Size":6300000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39760.jpg","Size":482345,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Mount Holyoke College"],"Contributors":["Dee Lauzon","Sue Lancelle","Marian Rice"]},"BIOLOGICALPROCESS":{"onto_name":"atrial cardiac muscle tissue morphogenesis","onto_id":"GO:0055009"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of the periphery of an atria mouse cardiac muscle.  This micrograph shows how rich cardiac tissue is with mitochondria reflecting its greater dependence on cellular respiration for ATP.  Interspersed within the mitochondria are lipid droplets which have been found to play an important role as energy source as well.  The Sarcoplasmic Reticulum consists of a consistent three-dimensional tubular system at the cells periphery and functions mainly to store large amounts of calcium ions which are sequestered until stimulated by an electrical impulse."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"sarcoplasmic reticulum","onto_id":"GO:0016529"},{"onto_name":"mitochondrion","onto_id":"GO:0005739"},{"onto_name":"lipid particle","onto_id":"GO:0005811"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"10969","TECHNICALDETAILS":{"free_text":"Primary fixation included: 2.5 % glutaraldehyde, 2% formaldehyde in  0.1 M Na-phosphate buffer, pH 7.4. Post-fixed in 2% OSO4 in 0.1 M Na-phosphate buffer, pH 7.4. Stained en bloc in 1% uranyl acetate. The tissue was then dehydrated in a graded series of ethanol and infiltrated with Spurr\u2019s resin.  Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph image was taken using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV."},"TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1120,"axis":"X"}},{"Space":{"Image_size":1846,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Mus musculus","onto_id":"NCBITaxon:10090"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"free_text":"ethanol dehydration"},{"onto_name":"tissue in epoxy resin embedment","onto_id":"FBbi:00000018"}],"VISUALIZATIONMETHODS":[{"onto_name":"uranyl salt","onto_id":"FBbi:00000569"},{"onto_name":"lead salt","onto_id":"FBbi:00000570"}],"CELLTYPE":{"onto_name":"cardiac muscle cell","onto_id":"CL:0000746"}}},"Citation":{"Title":"Dee Lauzon, Sue Lancelle, Marian Rice (2012) CIL:39760, Mus musculus, cardiac muscle cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39760","DOI":"doi:10.7295\/W9CIL39760"}}}