{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305172800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"25380.jpg","Size":33648,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"25380.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"25380.zip","Size":168610,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"Contributors":["El-Desouky Ammar","Saskia A. Hogenhout","L. R. Nault"]},"BIOLOGICALPROCESS":{"onto_name":"viral transmission by vector","onto_id":"GO:0044009"},"PROCESSINGHISTORY":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Aggregates of maize mosaic virus (MMV, Rhabdoviridae) in cells of the anterior diverticulum of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green\/yellow fluorescence) that accumulate mainly around the nuclei (red) in cells of the anterior diverticulum.  Accompanying image in this group contains a scale bar.\n\nMethods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP).\n\nAssociated references: \nAmmar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85.\nAmmar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"host cell perinuclear region of cytoplasm","onto_id":"GO:0044220"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},{"onto_name":"distribution of epitope","onto_id":"FBbi:00000592"}],"GROUP_ID":"8075","DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":354,"Pixel_size":{"unit":"microns","value":0.555},"axis":"X"}},{"Space":{"Image_size":253,"Pixel_size":{"unit":"microns","value":0.555},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":[{"onto_name":"Maize mosaic virus","onto_id":"NCBITaxon:279896"},{"onto_name":"Peregrinus maidis","onto_id":"NCBITaxon:222432"}],"PREPARATION":[{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},{"onto_name":"permeabilized tissue","onto_id":"FBbi:00000093"}],"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Alexa Fluor 488","onto_id":"FBbi:00000440"},{"onto_name":"propidium iodide","onto_id":"FBbi:00000047"}]}},"Citation":{"Title":"El-Desouky Ammar, Saskia A. Hogenhout, L. R. Nault (2011) CIL:25380, Maize mosaic virus, Peregrinus maidis. CIL. Dataset","ARK":"ark:\/b7295\/w9cil25380","DOI":"doi:10.7295\/W9CIL25380"},"Microbial_type":["virus"]}}