{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329022800},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39733.tif","Size":600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39733.jpg","Size":55978,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"39733.zip","Size":564726,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"Proceedings National Academy of sciences USA","Href":"http:\/\/www.pnas.org\/content\/103\/17\/6506.long"}],"PUBLISHED":["P.J. Robinson et al. 2006. EM measurements define the dimensions of the \"30-nm\" chromatin fiber: Evidence for a compact, interdigitated structure Proc Natl Acad Sci USA 103:6506-6511"],"Contributors":["Philip J Robinson","Louise Fairall","Van A Huynh","Daniela Rhodes"],"PUBMED":["16617109"]},"BIOLOGICALPROCESS":[{"onto_name":"chromatin organization","onto_id":"GO:0006325"},{"free_text":"DNA organization"}],"PROCESSINGHISTORY":{"onto_name":"montage","onto_id":"FBbi:00000261"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"EM images of chromatin fibers reconstituted from tandem arrays of a strong nucleosome positioning sequence flanked by 'linker' DNA, creating fibers containing 22 nucleosomes and  a nucleosome repeat length (NRL) of 177 bp. Reconstitutes had saturating amounts of purified core histones and linker histones. (A) shows a montage of 'end on' views of fibers obtained by cryo-fixation and recorded in the frozen hydrated state.  (B and C) contain views of 3D models considered to correspond to the images at left. See Fig 1 in P.J. Robinson et al. 2006. EM measurements define the dimensions of the \"30-nm\" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511."},"ITEMTYPE":[{"onto_name":"recorded image","onto_id":"FBbi:00000265"},{"onto_name":"portrayed image","onto_id":"FBbi:00000266"}],"CELLULARCOMPONENT":{"onto_name":"chromatin","onto_id":"GO:0000785"},"RELATIONTOINTACTCELL":{"onto_name":"isolated subcellular component","onto_id":"FBbi:00000579"},"SOURCEOFCONTRAST":{"onto_name":"intrinsic mass distribution","onto_id":"FBbi:00000607"},"GROUP_ID":"10718","TECHNICALDETAILS":{"free_text":"See: P.J. Robinson et al. 2006. EM measurements define the dimensions of the \"30-nm\" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":514,"axis":"X"}},{"Space":{"Image_size":366,"axis":"Y"}}],"PREPARATION":{"onto_name":"cryofixed tissue","onto_id":"FBbi:00000013"},"VISUALIZATIONMETHODS":{"onto_name":"visualization of contiguous regions","onto_id":"FBbi:00000396"}}},"Citation":{"Title":"Philip J Robinson, Louise Fairall, Van A Huynh, Daniela Rhodes (2012) CIL:39733. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39733","DOI":"doi:10.7295\/W9CIL39733"}}}