Cammer, Michael (2021). CIL:35283, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0D50KWK

The f-actin staining in the lamella of a cultured fibroblast shows common features of adherent cells on a flat substrate. The dominant f-actin organization is in the form of stress fibers, linear bundles of actin filaments ending at cell adhesions to the substrate. In the upper left, actin can be seen modeling the highly dynamic leading edge. There are three patches of ruffles behind the leading edge.

• 2021

• Cammer, Michael

Prepared slide purchased from Molecular Probes imaged with a 60X N.A. 1.4 phase 3 lens on an Olympus IX 70 microscope with a 100W Hg arc lamp for illumination, narrow pass filter blocks for widefield fluorescence microscopy, and a 1.5X relay lens in front of a Cooke Sensicam HQ air cooled CCD camera. Imaged at the Analytical Imaging Facility of the Albert Einstein College of Medicine.

Preparation: chemically fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Fluorescein (FITC); phalloidin
Data qualification: Processed;spatialmeasurements

• Mus musculus

• Fibroblast
• Lamellipodium
• Ruffle
• Stress fiber

• Interphase
• NIH/3T3

• data
• image

• No linguistic content; Not applicable

Samplenumber: 35283

Doi: https://doi.org/10.6075/J0D50KWK

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL35283

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2022-08-12