{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1329800400},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"39697.tif","Size":12700000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"39697.jpg","Size":910317,"Mime_type":"image\/jpeg; charset=utf-8"}],"CORE":{"ATTRIBUTION":{"OTHER":["Princeton EM Core Facility"],"Contributors":["Ethan Perlstein","Daniel Korostyshevsky"]},"BIOLOGICALPROCESS":[{"free_text":"sertraline treatment"},{"onto_name":"vacuole organization","onto_id":"GO:0007033"},{"onto_name":"vesicle fusion with vacuole","onto_id":"GO:0051469"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"PARAMETERIMAGED":{"onto_name":"electron density","onto_id":"FBbi:00000315"},"IMAGEDESCRIPTION":{"free_text":"Transmission electron micrograph of an unbudded yeast cell treaated with 60µM sertraline. The vacuolar contents are more electron-lucent, and vacuolar membrane is more discernible. A single lipid droplet is fusing with the lower large vacuole located left of center. The image contributor asks Library users to use the comments box to answer what is the electron-lucent material located underneath or abutting the vacuolar membranes?"},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"vacuole","onto_id":"GO:0005773"},{"onto_name":"lipid particle","onto_id":"GO:0005811"},{"onto_name":"nucleus","onto_id":"GO:0005634"}],"RELATIONTOINTACTCELL":{"onto_name":"sectioned tissue","onto_id":"FBbi:00000026"},"SOURCEOFCONTRAST":{"onto_name":"differences in adsorption or binding of stain","onto_id":"FBbi:00000598"},"GROUP_ID":"11310","TECHNICALDETAILS":{"free_text":"BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c.  Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X.  All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase.  This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes."},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"transmission electron microscopy (TEM)","onto_id":"FBbi:00000258"},"DIMENSION":[{"Space":{"Image_size":1992,"axis":"X"}},{"Space":{"Image_size":2110,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":[{"onto_name":"glutaraldehyde fixed tissue","onto_id":"FBbi:00000011"},{"onto_name":"tissue in plastic embedment","onto_id":"FBbi:00000016"}],"VISUALIZATIONMETHODS":{"onto_name":"osmium tetroxide","onto_id":"FBbi:00000571"},"CELLTYPE":{"free_text":"BY4716"}}},"Citation":{"Title":"Ethan Perlstein, Daniel Korostyshevsky (2012) CIL:39697, Saccharomyces cerevisiae, BY4716. CIL. Dataset","ARK":"ark:\/b7295\/w9cil39697","DOI":"doi:10.7295\/W9CIL39697"}}}