{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1305172800},"Data_type":{"Still_image":false,"Z_stack":true,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"31262.jpg","Size":78971,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"31262.tif","Size":29600000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"31262.zip","Size":29529190,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/3111\/"}],"PUBLISHED":["J Cell Biol. 2010. 190: 991-1003."],"Contributors":["Claudio R. Thoma","Alexandre Matov","Katrin L. Gutbrodt","Christian R. Hoerner","Zlatko Smole","Wilhelm Krek","Gaudenz Danuser"],"PUBMED":["20855504"]},"BIOLOGICALPROCESS":{"onto_name":"protein polymerization","onto_id":"GO:0051258"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"RCC4","onto_id":"MCC:0000395"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"RCC-4 cell stained with anti-alpha-tubulin (green) and anti-GTP-tubulin (red) antibodies. GTP-tubulin localizes to the microtubule (MT) tip and remnants of GTP-tubulin are found along the entire MT lattice. RCC-4 cells lack Von Hippel-Lindau (VHL) and when pVHL30 is expressed in these cells, a higher frequency of GTP caps and GTP remnants is observed. RCC-4 cells were permeabilized in PEM buffer with glycerol and 0.1% Triton-X100, then incubated with primary antibody hMB11 followed by incubation with secondary antibody. Cells were then fixed in cold methanol for 5 min and stained with the polyclonal anti-tubulin antibody followed by secondary antibody. Stacks of images were acquired with an IX70 Delta Vision Spectris microscope using a 60X NA1.4 DIC oil Plan-Apochromat objective. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--control Fig 5F (left 2 panels) and the experimental with ectopic pVHL30 (right 2 panels in Fig 5F) is CIL# 26572."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"cytoskeleton","onto_id":"GO:0005856"},{"onto_name":"protein complex","onto_id":"GO:0043234"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"8073","MOLECULARFUNCTION":[{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"structural molecule activity","onto_id":"GO:0005198"},{"onto_name":"protein binding","onto_id":"GO:0005515"}],"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},"DIMENSION":[{"Space":{"Image_size":960,"Pixel_size":{"unit":"microns","value":0.1103},"axis":"X"}},{"Space":{"Image_size":960,"Pixel_size":{"unit":"microns","value":0.1103},"axis":"Y"}},{"Space":{"Image_size":8,"Pixel_size":{"unit":"microns","value":0.2},"axis":"Z"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":[{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},{"onto_name":"detergent permeabilized","onto_id":"FBbi:00000262"}],"VISUALIZATIONMETHODS":{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},"CELLTYPE":[{"free_text":"renal cell carcinoma"},{"onto_name":"epithelial cell","onto_id":"CL:0000066"}]}},"Citation":{"Title":"Claudio R. Thoma, Alexandre Matov, Katrin L. Gutbrodt, Christian R. Hoerner, Zlatko Smole, Wilhelm Krek, Gaudenz Danuser (2011) CIL:31262, Homo sapiens, epithelial cell, renal cell carcinoma. CIL. Dataset","ARK":"ark:\/b7295\/w9cil31262","DOI":"doi:10.7295\/W9CIL31262"}}}