{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1317441600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"38598.tif","Size":84600000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"38598.jpg","Size":3380849,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"38598.zip","Size":84461471,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"article","Href":"http:\/\/www.pnas.org\/content\/104\/51\/20308.long"}],"PUBLISHED":["Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220."],"Contributors":["Hari Shroff","Catherine G Galbraith","James A. Galbraith","Helen White","Jennifer Gillette","Scott Olenych","Michael W. Davidson","Eric Betzig"],"PUBMED":["18077327"]},"BIOLOGICALPROCESS":[{"free_text":"molecular organization of focal adhesion components"},{"onto_name":"focal adhesion assembly","onto_id":"GO:0048041"}],"PROCESSINGHISTORY":{"free_text":"probability density map"},"CELLLINE":{"free_text":"foreskin fibroblast"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Photoactivation localization microscopy image (PALM) of a human foreskin fibroblast expressing Dronpa-alpha-actinin (red) and tdEos-vinculin (green).  This image reveals that although conventional diffraction limited microscopy indicates alpha-actinin and vinculin colocalize in focal adhesions, PALM clearly shows that they these molecules are spatially segregated.  The DIC corresponds to the same image field is CIL 38597 and the diffraction limited TIRF image is CIL 38599. Bar is 2 microns.  Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNASi U S A. 2007 Dec 18;104(51):20308-13."},"ITEMTYPE":{"onto_name":"portrayed image","onto_id":"FBbi:00000266"},"CELLULARCOMPONENT":[{"onto_name":"focal adhesion","onto_id":"GO:0005925"},{"onto_name":"actin cytoskeleton","onto_id":"GO:0015629"},{"free_text":"alpha-actinin"},{"free_text":"vinculin"}],"RELATIONTOINTACTCELL":{"onto_name":"dispersed cells in vitro","onto_id":"FBbi:00000611"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"9553","TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":{"onto_name":"PALM (photoactivation localization microscopy)","onto_id":"FBbi:00000335"},"DIMENSION":[{"Space":{"Image_size":5787,"axis":"X"}},{"Space":{"Image_size":4865,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"Dronpa","onto_id":"FBbi:00000558"},{"free_text":"tdEos"}],"CELLTYPE":{"onto_name":"fibroblast","onto_id":"CL:0000057"}}},"Citation":{"Title":"Hari Shroff, Catherine G Galbraith, James A. Galbraith, Helen White, Jennifer Gillette, Scott Olenych, Michael W. Davidson, Eric Betzig (2011) CIL:38598, Homo sapiens, fibroblast. CIL. Dataset","ARK":"ark:\/b7295\/w9cil38598","DOI":"doi:10.7295\/W9CIL38598"}}}