{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1307505600},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"13865.jpg","Size":9234,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"13865.tif","Size":300000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"13865.zip","Size":214682,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Href":"http:\/\/jcb-dataviewer.rupress.org\/jcb\/browse\/4053\/"}],"PUBLISHED":["J Cell Biol. (2011) 192: 599-614."],"Contributors":["Mauricio Valerio-Santiago","Fernando Monje-Casas"],"PUBMED":["21321099"]},"BIOLOGICALPROCESS":[{"onto_name":"cell cycle","onto_id":"GO:0007049"},{"onto_name":"protein dephosphorylation","onto_id":"GO:0006470"},{"onto_name":"regulation of exit from mitosis","onto_id":"GO:0007096"},{"onto_name":"mitotic sister chromatid segregation","onto_id":"GO:0000070"},{"onto_name":"microtubule-based process","onto_id":"GO:0007017"}],"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"free_text":"W303"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"3HA-Cdc14 (red) localization in anaphase. The protein is released throughout the whole cell. Anaphase is determined by spindle morphology (tubulin, green) and nuclear morphology (DAPI, blue). Image is Fig 3D, right panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 3 include CIL# 13862, 13863, 13864, 13865, 13866, 13867."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"spindle pole body","onto_id":"GO:0005816"},{"onto_name":"nucleus","onto_id":"GO:0005634"},{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"spindle pole body","onto_id":"GO:0005816"},{"onto_name":"tubulin complex","onto_id":"GO:0045298"},{"onto_name":"cytoplasm","onto_id":"GO:0005737"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":[{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},{"onto_name":"compartmentalization of stain or label","onto_id":"FBbi:00000604"}],"GROUP_ID":"9034","MOLECULARFUNCTION":[{"onto_name":"protein tyrosine\/serine\/threonine phosphatase activity","onto_id":"GO:0008138"},{"onto_name":"GTP binding","onto_id":"GO:0005525"},{"onto_name":"GTPase activity","onto_id":"GO:0003924"},{"onto_name":"nucleotide binding","onto_id":"GO:0000166"},{"onto_name":"structural constituent of cytoskeleton","onto_id":"GO:0005200"}],"TECHNICALDETAILS":{"free_text":"Cells (MATa cdc14::3HA-CDC14), grown in rich media with glucose, were fixed for 15 min in 3.7% formaldehyde and 0.1 M potassium phosphate buffer, pH 6.4. Cells were then washed twice with 0.1 M potassium phosphate buffer, pH 6.4, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Fixed cells were digested with 0.1 mg\/ml zymolyase-100T (US Biological) and 1\/10 volume of glusulase (PerkinElmer) at 30C for 15 min, washed once, and resuspended in 1.2 M sorbitol in 0.12 M K2HPO4\/0.033 M citric acid, pH 5.9. Primary antibodies were anti-HA monoclonal antibody (HA.11; 1:500) and anti-tubulin (Abcam; 1:500). Secondary antibodies were: anti-mouse Cy3 (for HA) and anti-rat FITC (for tubulin). Cells were resuspended in DAPI (1 mg\/ml). Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x\/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software."},"DATAQUALIFICATION":{"free_text":"RAW;spatialmeasurements;intensitiesquantitation"},"TERMSANDCONDITIONS":{"free_text":"attribution_nc_sa"},"IMAGINGMODE":[{"onto_name":"widefield illumination","onto_id":"FBbi:00000277"},{"onto_name":"fluorescence microscopy","onto_id":"FBbi:00000246"}],"DIMENSION":[{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"X"}},{"Space":{"Image_size":187,"Pixel_size":{"unit":"microns","value":0.0642},"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Saccharomyces cerevisiae","onto_id":"NCBITaxon:4932"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"HA peptide tag","onto_id":"FBbi:00000034"},{"onto_name":"4',6-diamidino-2-phenylindole (DAPI)","onto_id":"FBbi:00000056"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Cy3","onto_id":"FBbi:00000449"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"}]}},"Citation":{"Title":"Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13865, Saccharomyces cerevisiae. CIL. Dataset","ARK":"ark:\/b7295\/w9cil13865","DOI":"doi:10.7295\/W9CIL13865"}}}