{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1286251200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"Jpeg","File_path":"7238.jpg","Size":85412,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"OME_tif","File_path":"7238.tif","Size":3100000,"Mime_type":"image\/tif"},{"File_type":"Zip","File_path":"7238.zip","Size":3020374,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"OTHER":["Tim Mitchison Lab"],"Contributors":["Hu, Chi-Kuo"]},"BIOLOGICALPROCESS":{"onto_name":"cytokinesis","onto_id":"GO:0000910"},"PROCESSINGHISTORY":{"onto_name":"unprocessed raw data","onto_id":"FBbi:00000582"},"CELLLINE":{"onto_name":"HeLa","onto_id":"MCC:0000219"},"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"HeLa cells were accumulated in monopolar mitosis using a 12 hr treatment of the kinesin-5 inhibitor S-trityl-l-cysteine and forced into cytokinesis by adding the potent Cdk1 inhibitor purvalanol A for 15 min.  The microtubules (green) were not growing and shrinking.  Aurora B (red), which functions in attaching microtubules to the centromere, localizes at the gap region between the cortex and the microtubule plus ends.\n\nCells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against Auruoa B and FITC-DM1a antibody against microtubules.  Secondary antibody was Alexa 594 for Aurora B.   A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective.  Images were collected with an Orca ER CCD camera.\nLasers and filters:  Innova 70C Spectrum 3 watt Laser, 488 Laser\/ATOF 525\/50, and 568 Laser\/ATOF 605\/52."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"microtubule","onto_id":"GO:0005874"},{"onto_name":"chromosome passenger complex","onto_id":"GO:0032133"}],"RELATIONTOINTACTCELL":{"onto_name":"whole mounted tissue","onto_id":"FBbi:00000024"},"SOURCEOFCONTRAST":{"onto_name":"distribution of a specific protein","onto_id":"FBbi:00000597"},"GROUP_ID":"1454","TERMSANDCONDITIONS":{"free_text":"public_domain"},"IMAGINGMODE":{"onto_name":"spinning disk confocal microscopy","onto_id":"FBbi:00000253"},"DIMENSION":[{"Space":{"Image_size":1114,"Pixel_size":{"unit":"microns","value":0.064},"axis":"X"}},{"Space":{"Image_size":901,"Pixel_size":{"unit":"microns","value":0.064},"axis":"Y"}},{"Wavelength":{"unit":"nanometers","value":"488"}},{"Wavelength":{"unit":"nanometers","value":"568"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Homo sapiens","onto_id":"NCBITaxon:9606"},"PREPARATION":{"onto_name":"methanol fixed tissue","onto_id":"FBbi:00000007"},"VISUALIZATIONMETHODS":[{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"},{"onto_name":"Fluorescein (FITC)","onto_id":"FBbi:00000451"},{"onto_name":"Alexa Fluor 594","onto_id":"FBbi:00000444"},{"onto_name":"labeled primary antibody","onto_id":"FBbi:00000130"}],"CELLTYPE":{"onto_name":"epithelial cell","onto_id":"CL:0000066"}}},"Citation":{"Title":"Hu, Chi-Kuo (2010) CIL:7238, Homo sapiens, epithelial cell. CIL. Dataset","ARK":"ark:\/b7295\/w9cil7238","DOI":"doi:10.7295\/W9CIL7238"}}}