{"CIL_CCDB":{"Status":{"Is_public":true,"Deleted":false,"Publish_time":1316491200},"Data_type":{"Still_image":true,"Z_stack":false,"Video":false,"Time_series":false},"CIL":{"Image_files":[{"File_type":"OME_tif","File_path":"36433.tif","Size":3400000,"Mime_type":"image\/tif"},{"File_type":"Jpeg","File_path":"36433.jpg","Size":271299,"Mime_type":"image\/jpeg; charset=utf-8"},{"File_type":"Zip","File_path":"36433.zip","Size":2077304,"Mime_type":"application\/zip"}],"CORE":{"ATTRIBUTION":{"URLs":[{"Label":"CCDB 75","Href":"http:\/\/ccdb.ucsd.edu\/sand\/main?event=displayAll&mpid=75"}],"Contributors":["Eric A. Bushong","Maryann E. Martone","Mark H. Ellisman"],"PUBMED":["PMID:15036382"]},"BIOLOGICALPROCESS":[{"onto_name":"forebrain astrocyte development","onto_id":"GO:0021897"},{"onto_name":"cell projection organization","onto_id":"GO:0030030"}],"PARAMETERIMAGED":{"onto_name":"fluorescence emission","onto_id":"FBbi:00000316"},"IMAGEDESCRIPTION":{"free_text":"Intracellular dye injections of protoplasmic a 1-week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow (green) and immunostained to reveal the localization of glial-specific proteins S-100 (red) and GFAP (blue).  Optical sections were generated with a single photon confocal microscope. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database."},"ITEMTYPE":{"onto_name":"recorded image","onto_id":"FBbi:00000265"},"CELLULARCOMPONENT":[{"onto_name":"cell projection cytoplasm","onto_id":"GO:0032838"},{"onto_name":"cell body","onto_id":"GO:0044297"}],"RELATIONTOINTACTCELL":{"onto_name":"vibratome-sectioned tissue","onto_id":"FBbi:00000030"},"SOURCEOFCONTRAST":{"onto_name":"intracellular filling","onto_id":"FBbi:00000088"},"GROUP_ID":"11050","TECHNICALDETAILS":{"free_text":"Male Sprague Dawley rats were anesthetized and perfused transcardially with with Ringer\u2019s solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4).  Tissue was sectioned (100 µm thick) using a vibratome, and injected  with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7\u201311µm) in the stratum\nradiatum of CA1 were located and impaled with glass micropipettes filled with either 5% aq. lucifer yellow. The astrocytes were filled using a 0.5 Hz pulses of\ncurrent until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C, and subsequently immunostained. Slices were then imaged after coverslipping in Gelvatol.  Confocal images were acquired with a BioRad Radiance2000\n(Hercules, CA) attached to a Nikon E600FN\n(Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60× oil objective (NA 1.4). Optical z resolution is 0.25µm. Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86."},"DATAQUALIFICATION":{"free_text":"PROCESSED"},"TERMSANDCONDITIONS":{"free_text":"attribution_cc_by"},"IMAGINGMODE":{"onto_name":"confocal microscopy","onto_id":"FBbi:00000251"},"DIMENSION":[{"Space":{"Image_size":1052,"axis":"X"}},{"Space":{"Image_size":1052,"axis":"Y"}}],"NCBIORGANISMALCLASSIFICATION":{"onto_name":"Rattus norvegicus","onto_id":"NCBITaxon:10116"},"PREPARATION":{"onto_name":"formaldehyde fixed tissue","onto_id":"FBbi:00000010"},"VISUALIZATIONMETHODS":[{"onto_name":"lucifer yellow","onto_id":"FBbi:00000057"},{"onto_name":"Alexa Fluor 568","onto_id":"FBbi:00000442"},{"onto_name":"X-Rhodamine","onto_id":"FBbi:00000584"},{"onto_name":"primary antibody plus labeled secondary antibody","onto_id":"FBbi:00000156"}],"CELLTYPE":[{"onto_name":"astrocyte of the hippocampus","onto_id":"CL:0002604"},{"onto_name":"astrocyte","onto_id":"CL:0000127"}]}},"Citation":{"Title":"Eric A. Bushong, Maryann E. Martone, Mark H. Ellisman (2011) CIL:36433, Rattus norvegicus, astrocyte of the hippocampus, astrocyte. CIL. Dataset","ARK":"ark:\/b7295\/w9cil36433","DOI":"doi:10.7295\/W9CIL36433"}}}